BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4344

Title: 1H and 15N Chemical Shift Assignments for Apo-CopZ

Authors: Wimmer, Reinhard; Herrmann, Torsten; Solioz, Marc; Wuethrich, Kurt

Citation: Wimmer, Reinhard; Herrmann, Torsten; Solioz, Marc; Wuethrich, Kurt. "NMR Structure and Metal Interactions of the CopZ Copper Chaperone"  J. Biol. Chem. ., .-..

Assembly members:
CopZ, polymer, 68 residues, Formula weight is not available

Natural source:   Common Name: Enterococcus hirae   Taxonomy ID: 1354   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Enterococcus hirae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CopZ: AQEFSVKGMSCNHCVARIEE AVGRISGVKKVKVQLKKEKA VVKFDEANVQATEICQAINE LGYQAEVI

Data sets:
Data typeCount
15N chemical shifts74
1H chemical shifts443

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo CopZ monomer1

Entities:

Entity 1, apo CopZ monomer 68 residues - Formula weight is not available

1   ALAGLNGLUPHESERVALLYSGLYMETSER
2   CYSASNHISCYSVALALAARGILEGLUGLU
3   ALAVALGLYARGILESERGLYVALLYSLYS
4   VALLYSVALGLNLEULYSLYSGLULYSALA
5   VALVALLYSPHEASPGLUALAASNVALGLN
6   ALATHRGLUILECYSGLNALAILEASNGLU
7   LEUGLYTYRGLNALAGLUVALILE

Samples:

sample_1: CopZ, [U-15N], 1.3 mM; sodium phosphate 50 mM; Na2S2O4 10 uM

conditions_1: pH: 7.0 na; temperature: 288.2 K

Experiments:

NameSampleSample stateSample conditions
2D 2QF-COSYnot availablenot availablenot available
2D 3QF-COSYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D ct-HNHBnot availablenot availablenot available
mixing time of NOESY spectra: 50 msnot availablenot availablenot available
mixing time of TOCSY spectra: 50 and 90 msnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz

Related Database Links:

PDB
EMBL CAA86836
GB AFM70728 EOF61613 EOH69882 EOU07149 KDR91397
REF WP_010718490
SP Q47840