BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4348

Title: 1H, 15N and 13C Chemical Shift Assignments for NtrC-C-term-3Ala

Authors: Pelton, Jeffrey; Kustu, Sydney; Wemmer, David

Citation: Pelton, Jeffrey; Kustu, Sydney; Wemmer, David. "Solution Structure of the DNA-binding Domain of NtrC with Three Alanine Substitutions"  J. Mol. Biol. ., .-..

Assembly members:
Nitrogen regulatory protein C, polymer, 91 residues, 19962 Da.

Natural source:   Common Name: Salmonella typhimurium   Taxonomy ID: 602   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Salmonella typhimurium

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Nitrogen regulatory protein C: MDLPGELFEASTPDSPSHLP PDSWATLLAQWADRALRSGH QNLLSEAQPELERTLLTTAL RHTQGHKQEAARLLGWGAAT LTAKLKELGME

Data sets:
Data typeCount
13C chemical shifts281
15N chemical shifts77
1H chemical shifts430

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NtrC-C-term-3Ala subunit 11
2NtrC-C-term-3Ala subunit 21

Entities:

Entity 1, NtrC-C-term-3Ala subunit 1 91 residues - 19962 Da.

1   METASPLEUPROGLYGLULEUPHEGLUALA
2   SERTHRPROASPSERPROSERHISLEUPRO
3   PROASPSERTRPALATHRLEULEUALAGLN
4   TRPALAASPARGALALEUARGSERGLYHIS
5   GLNASNLEULEUSERGLUALAGLNPROGLU
6   LEUGLUARGTHRLEULEUTHRTHRALALEU
7   ARGHISTHRGLNGLYHISLYSGLNGLUALA
8   ALAARGLEULEUGLYTRPGLYALAALATHR
9   LEUTHRALALYSLEULYSGLULEUGLYMET
10   GLU

Samples:

sample_1: Nitrogen regulatory protein C, [U-98% 13C; U-95% 15N], 0.5 – 1.5 mM; sodium_phosphate 20 mM; potassium_chloride 50 mM; EDTA 0.1 mM; sodium_azide 0.02 w/v %

Ex-cond_1: pH: 6.1; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HCACOnot availablenot availablenot available
HNCOnot availablenot availablenot available
HA(CO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker DRX 500 MHz

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