BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4384

Title: Solution Structure and Dynamics of the Rous Sarcoma Virus Capsid Protein and Comparison with Capsid Proteins ofOther Retroviruses   PubMed: 10677830

Authors: Campos-Olivas, Ramon; Newman, John; Summers, Michael

Citation: Campos-Olivas, Ramon; Newman, John; Ndassa, Y.; Summers, Michael. "1H, 13C, and 15N Chemical Shift Assignments of the Capsid Protein from Rous Sarcoma Virus"  J. Biomol. NMR 15, 267-268 (1999).

Assembly members:
Capsid protein from RSV, polymer, 262 residues, 28401 Da.

Natural source:   Common Name: Rous Sarcoma Virus   Taxonomy ID: 11889   Superkingdom: not available   Kingdom: not available   Genus/species: Avian type C retroviruses Rous Sarcoma Virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Capsid protein from RSV: GHHHHHHHHHHSSGHIEGRH NMPVVIKTEGPAWTPLEPKL ITRLADTVRTKGLRSPITMA EVEALMSSPLLPHDVTNLMR VILGPAPYALWMDAWGVQLQ TVIAAATRDPRHPANGQGRG ERTNLDRLKGLADGMVGNPQ GQAALLRPGELVAITASALQ AFREVARLAEPAGPWADITQ GPSESFVDFANRLIKAVEGS DLPPSARAPVIIDCFRQKSQ PDIQQLIRAAPSTLTTPGEI IKYVLDRQKIAPLTDQGIAA AM

Data sets:
Data typeCount
13C chemical shifts1067
15N chemical shifts241
1H chemical shifts1595

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CA RSV1

Entities:

Entity 1, CA RSV 262 residues - 28401 Da.

1   GLYHISHISHISHISHISHISHISHISHIS
2   HISSERSERGLYHISILEGLUGLYARGHIS
3   ASNMETPROVALVALILELYSTHRGLUGLY
4   PROALATRPTHRPROLEUGLUPROLYSLEU
5   ILETHRARGLEUALAASPTHRVALARGTHR
6   LYSGLYLEUARGSERPROILETHRMETALA
7   GLUVALGLUALALEUMETSERSERPROLEU
8   LEUPROHISASPVALTHRASNLEUMETARG
9   VALILELEUGLYPROALAPROTYRALALEU
10   TRPMETASPALATRPGLYVALGLNLEUGLN
11   THRVALILEALAALAALATHRARGASPPRO
12   ARGHISPROALAASNGLYGLNGLYARGGLY
13   GLUARGTHRASNLEUASPARGLEULYSGLY
14   LEUALAASPGLYMETVALGLYASNPROGLN
15   GLYGLNALAALALEULEUARGPROGLYGLU
16   LEUVALALAILETHRALASERALALEUGLN
17   ALAPHEARGGLUVALALAARGLEUALAGLU
18   PROALAGLYPROTRPALAASPILETHRGLN
19   GLYPROSERGLUSERPHEVALASPPHEALA
20   ASNARGLEUILELYSALAVALGLUGLYSER
21   ASPLEUPROPROSERALAARGALAPROVAL
22   ILEILEASPCYSPHEARGGLNLYSSERGLN
23   PROASPILEGLNGLNLEUILEARGALAALA
24   PROSERTHRLEUTHRTHRPROGLYGLUILE
25   ILELYSTYRVALLEUASPARGGLNLYSILE
26   ALAPROLEUTHRASPGLNGLYILEALAALA
27   ALAMET

Samples:

sample_1: Capsid protein from RSV, [U-13C; U-15N], 1.0 mM; H2O0 – 90%; D2O10 – 100%; phosphate 10 mM; N3Na 0.2 mM; EDTA 0.1 mM; PMSF 0.1 mM; pepstatin A 1 mg/L; beta-mercaptoethanol 5 mM

sample_2: Capsid protein from RSV, [U-15N], 1.0 mM; H2O0 – 90%; D2O10 – 100%; phosphate 10 mM; N3Na 0.2 mM; EDTA 0.1 mM; PMSF 0.1 mM; pepstatin A 1 mg/L; beta-mercaptoethanol 5 mM

sample_3: Capsid protein from RSV, [U-80% 2H; U-13C; U-15N], 1.0 mM; H2O0 – 90%; D2O10 – 100%; phosphate 10 mM; N3Na 0.2 mM; EDTA 0.1 mM; PMSF 0.1 mM; pepstatin A 1 mg/L; beta-mercaptoethanol 5 mM

sample_4: Capsid protein from RSV, [U-80% 2H; U-15N], 1.0 mM; H2O0 – 90%; D2O10 – 100%; phosphate 10 mM; N3Na 0.2 mM; EDTA 0.1 mM; PMSF 0.1 mM; pepstatin A 1 mg/L; beta-mercaptoethanol 5 mM

sample_5: Capsid protein from RSV, [U-15N]-V, 1.0 mM; H2O0 – 90%; D2O10 – 100%; phosphate 10 mM; N3Na 0.2 mM; EDTA 0.1 mM; PMSF 0.1 mM; pepstatin A 1 mg/L; beta-mercaptoethanol 5 mM

sample_6: Capsid protein from RSV, [U-15N]-L, 1.0 mM; H2O0 – 90%; D2O10 – 100%; phosphate 10 mM; N3Na 0.2 mM; EDTA 0.1 mM; PMSF 0.1 mM; pepstatin A 1 mg/L; beta-mercaptoethanol 5 mM

sample_7: Capsid protein from RSV, [U-15N]-I, 1.0 mM; H2O0 – 90%; D2O10 – 100%; phosphate 10 mM; N3Na 0.2 mM; EDTA 0.1 mM; PMSF 0.1 mM; pepstatin A 1 mg/L; beta-mercaptoethanol 5 mM

sample_8: Capsid protein from RSV, [U-15N]-D,N,E,Q,S,F,Y,W,C, 1.0 mM; H2O0 – 90%; D2O10 – 100%; phosphate 10 mM; N3Na 0.2 mM; EDTA 0.1 mM; PMSF 0.1 mM; pepstatin A 1 mg/L; beta-mercaptoethanol 5 mM

sample_9: Capsid protein from RSV, [U-10% 13C; U-15N], 1.0 mM; H2O0 – 90%; D2O10 – 100%; phosphate 10 mM; N3Na 0.2 mM; EDTA 0.1 mM; PMSF 0.1 mM; pepstatin A 1 mg/L; beta-mercaptoethanol 5 mM

sample_conditions: pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAA01499 BAC65349 BAC78442 BAG16456 BAK64239
EMBL CAA24499 CAA24512 CAA32008 CAA36153 CAA48534
GB AAA19606 AAA19607 AAA19608 AAA42377 AAA42406
PIR FOFV29
PRF 2019512A 2019512B 2019512C
REF NP_045935 NP_056886 NP_056887 NP_955601 NP_955609
SP O92954 O92956 P03322 P03323 P03354