BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4423

Title: Solution structure of the N-terminal zinc finger of murine GATA-1   PubMed: 10212985

Authors: Kowalski, K.; Czolij, R.; King, G.; Crossley, M.; Mackay, J.

Citation: Kowalski, K.; Czolij, R.; King, G.; Crossley, M.; Mackay, J.. "The Solution Structure of the N-terminal Zinc Finger of GATA-1 Reveals a Specific Binding Face for the Transcriptional Co-factor FO"  J. Biomol. NMR 13, 249-262 (1999).

Assembly members:
GATA-1, polymer, 46 residues, 5189 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GATA-1: GSEARECVNCGATATPLWRR DRTGHYLCNACGLYHKMNGQ NRPLIR

Data sets:
Data typeCount
1H chemical shifts303
15N chemical shifts36
coupling constants20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GATA-11
2ZN2

Entities:

Entity 1, GATA-1 46 residues - 5189 Da.

1   GLYSERGLUALAARGGLUCYSVALASNCYS
2   GLYALATHRALATHRPROLEUTRPARGARG
3   ASPARGTHRGLYHISTYRLEUCYSASNALA
4   CYSGLYLEUTYRHISLYSMETASNGLYGLN
5   ASNARGPROLEUILEARG

Entity 2, ZN - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: GATA-1 4 mM; Tris 10 mM; TCEP 5 mM; ZnSO4 5 mM

sample_2: GATA-1, [U-15N], 2 mM; Tris 20 mM; TCEP 3 mM; ZnSO4 3 mM

sample_cond_1: pH: 5.4; temperature: 288 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
TOCSYnot availablenot availablesample_cond_1
DQFCOSYnot availablenot availablesample_cond_1
NOESYnot availablenot availablesample_cond_1
ECOSYnot availablenot availablesample_cond_1
HSQCnot availablenot availablesample_cond_1
TOCSY-HSQCnot availablenot availablesample_cond_1
NOESY-HSQCnot availablenot availablesample_cond_1
J-MODULATED HMQCnot availablenot availablesample_cond_1

Software:

XWINNMR - 1D/2D data processing

Aurelia - 3D data processing

DIANA - distance geometry

X-Plor - dynamical simulated-annealing

MOLMOL - molecular visualisation

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAG68610 BAK39708
GB AFX60070 EAW50750
REF XP_007507788 XP_008156485 XP_008695477 XP_009437329