BMRB Entry 10023

Title:
Chemical shift assignment of human eIF2alpha
Deposition date:
2006-08-01
Original release date:
2006-12-04
Authors:
Ito, Takuhiro; Wagner, Gerhard
Citation:

Citation: Ito, Takuhiro; Wagner, Gerhard. "Resonance assignments of the alpha subunit of human eukaryotic initiation factor 2 (heIF2alpha)"  J. Biomol. NMR 38, 173-173 (2007).
PubMed: 17122952

Assembly members:

Assembly members:
heIF2alpha, polymer, 308 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts910
15N chemical shifts282
1H chemical shifts753

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1se-heIF2alpha1

Entities:

Entity 1, se-heIF2alpha 308 residues - Formula weight is not available

1   METSERCYSARGPHETYRGLNHISLYSPHE
2   PROGLUVALGLUASPVALVALMETVALASN
3   VALARGSERILEGLNGLUMETGLYALATYR
4   VALSERLEULEUGLUTYRASNASNILEGLU
5   GLYMETILEHISLEUSERGLULEUSERARG
6   ARGARGILEARGSERILEASNLYSLEUILE
7   ARGILEGLYARGASNGLUCYSVALLYSVAL
8   ILEARGVALASPLYSGLULYSGLYTYRILE
9   ASPLEUSERLYSARGARGVALSERPROGLU
10   GLUALAILELYSCYSGLUASPLYSPHETHR
11   LYSSERLYSTHRVALTYRSERILELEUARG
12   HISVALALAGLUVALLEUGLUTYRTHRLYS
13   ASPGLUGLNLEUGLUSERLEUPHEGLNARG
14   THRALATRPVALPHEASPASPLYSTYRLYS
15   ARGPROGLYTYRGLYALATYRASPALAPHE
16   LYSHISALAVALSERASPPROSERILELEU
17   ASPSERLEUASPLEUASNGLUASPGLUARG
18   GLUVALLEUILEASNASNILEASNARGARG
19   LEUTHRPROGLNALAVALLYSILEARGALA
20   ASPILEGLUVALALACYSTYRGLYTYRGLU
21   GLYILEASPALAVALLYSGLUALALEUARG
22   ALAGLYLEUASNCYSSERTHRGLUASNMET
23   PROILELYSILEASNLEUILEALAPROPRO
24   ARGTYRVALMETTHRTHRTHRTHRLEUGLU
25   ARGTHRGLUGLYLEUSERVALLEUSERGLN
26   ALAMETALAVALILELYSGLULYSILEGLU
27   GLULYSARGGLYVALPHEASNVALGLNMET
28   GLUPROLYSVALVALTHRASPTHRASPGLU
29   THRGLULEUALAARGGLNMETGLUARGLEU
30   GLUARGGLUASNALAGLUVALASPGLYASP
31   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: heIF2alpha, [U-2H; U-13C; U-15N], 0.5 ± 0.05 mM

sample_2: heIF2alpha, [NA-I,L,V-Me; U-2H; 13C-I,L,V; U-15N], 0.5 ± 0.05 mM

sample_3: heIF2alpha, [NA-I,L,V-Me; NA-F,Y; U-2H; 13C-I,L,V-Me; U-15N], 0.5 ± 0.05 mM

sample_4: heIF2alpha, [NA-F; U-2H], 0.5 ± 0.05 mM

sample_5: heIF2alpha, [NA-Y; U-2H], 0.5 ± 0.05 mM

condition_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available
3D TROSY-HNCOnot availablenot availablenot available
3D TROSY-HN(CA)COnot availablenot availablenot available
3D TROSY-HNCAnot availablenot availablenot available
3D TROSY-HN(CO)CAnot availablenot availablenot available
3D TROSY-HN(CA)CBnot availablenot availablenot available
3D TROSY-HN(COCA)CBnot availablenot availablenot available
3D 15N-NOESY-HSQCnot availablenot availablenot available
3D TROSY-Me-H(CCO)NHnot availablenot availablenot available
3D TROSY-Me-C(CO)NHnot availablenot availablenot available
3D Me-HCCH-TOCSYnot availablenot availablenot available
3D Me-HMQC-NOESYnot availablenot availablenot available
4D Me-HMQC-NOESY-Me-HMQCnot availablenot availablenot available
2D NOESY for aromaticnot availablenot availablenot available
2D TOCSY for aromaticnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • CMR G 750 MHz
  • Varian Inova 750 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz
  • Varian Inova 500 MHz

Related Database Links:

PDB
DBJ BAB27049 BAE25400 BAE39796 BAE89774 BAG35351
EMBL CAA37728 CAD61953 CAH93423
GB AAA41110 AAA52373 AAH02513 AAH05463 AAH16448
REF NP_001127006 NP_001244395 NP_001270066 NP_004085 NP_062229
SP P05198 P68101 P68102 Q5R493 Q6ZWX6
TPG DAA25059
AlphaFold P05198 P68101 P68102 Q5R493 Q6ZWX6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks