BMRB Entry 15773
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15773
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Citation: Tettamanzi, M. Cristina; Keeler, Camille; Meshack, Syrus; Hodsdon, Michael. "Analysis of Site-specific Histidine Protonation in Human Prolactin" Biochemistry 47, 8638-8647 (2008).
PubMed: 18652486
Assembly members:
hPrl, polymer, 199 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT7L-hPrl
| Data type | Count |
| 13C chemical shifts | 34 |
| 15N chemical shifts | 47 |
| 1H chemical shifts | 53 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hPrl | 1 |
Entities:
Entity 1, hPrl 199 residues - Formula weight is not available
| 1 | LEU | PRO | ILE | CYS | PRO | GLY | GLY | ALA | ALA | ARG | ||||
| 2 | CYS | GLN | VAL | THR | LEU | ARG | ASP | LEU | PHE | ASP | ||||
| 3 | ARG | ALA | VAL | VAL | LEU | SER | HIS | TYR | ILE | HIS | ||||
| 4 | ASN | LEU | SER | SER | GLU | MET | PHE | SER | GLU | PHE | ||||
| 5 | ASP | LYS | ARG | TYR | THR | HIS | GLY | ARG | GLY | PHE | ||||
| 6 | ILE | THR | LYS | ALA | ILE | ASN | SER | CYS | HIS | THR | ||||
| 7 | SER | SER | LEU | ALA | THR | PRO | GLU | ASP | LYS | GLU | ||||
| 8 | GLN | ALA | GLN | GLN | MET | ASN | GLN | LYS | ASP | PHE | ||||
| 9 | LEU | SER | LEU | ILE | VAL | SER | ILE | LEU | ARG | SER | ||||
| 10 | TRP | ASN | GLU | PRO | LEU | TYR | HIS | LEU | VAL | THR | ||||
| 11 | GLU | VAL | ARG | GLY | MET | GLN | GLU | ALA | PRO | GLU | ||||
| 12 | ALA | ILE | LEU | SER | LYS | ALA | VAL | GLU | ILE | GLU | ||||
| 13 | GLU | GLN | THR | LYS | ARG | LEU | LEU | GLU | GLY | MET | ||||
| 14 | GLU | LEU | ILE | VAL | SER | GLN | VAL | HIS | PRO | GLU | ||||
| 15 | THR | LYS | GLU | ASN | GLU | ILE | TYR | PRO | VAL | TRP | ||||
| 16 | SER | GLY | LEU | PRO | SER | LEU | GLN | MET | ALA | ASP | ||||
| 17 | GLU | GLU | SER | ARG | LEU | SER | ALA | TYR | TYR | ASN | ||||
| 18 | LEU | LEU | HIS | CYS | LEU | ARG | ARG | ASP | SER | HIS | ||||
| 19 | LYS | ILE | ASP | ASN | TYR | LEU | LYS | LEU | LEU | LYS | ||||
| 20 | CYS | ARG | ILE | ILE | HIS | ASN | ASN | ASN | CYS |
Samples:
sample_1: hPrl, [U-100% 13C; U-100% 15N], 0.6 mM; potassium phosphate 25 mM; sodium chloride 25 mM; sodium azide 1 mM; H2O 95%; D2O 5%
sample_2: hPrl, [U-100% 13C; U-100% 15N], 0.6 mM; potassium phosphate 25 mM; sodium chloride 25 mM; sodium azide 1 mM; H2O 95%; D2O 5%
sample_3: hPrl, [U-100% 13C; U-100% 15N], 0.6 mM; potassium phosphate 25 mM; sodium chloride 25 mM; sodium azide 1 mM; H2O 95%; D2O 5%
sample_4: hPrl, [U-100% 13C; U-100% 15N], 0.6 mM; potassium phosphate 25 mM; sodium chloride 25 mM; sodium azide 1 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.050 M; pH: 5.0; pressure: 1 atm; temperature: 308 K
sample_conditions_2: ionic strength: 0.052 M; pH: 6.0; pressure: 1 atm; temperature: 308 K
sample_conditions_3: ionic strength: 0.064 M; pH: 7.0; pressure: 1 atm; temperature: 308 K
sample_conditions_4: ionic strength: 0.082 M; pH: 8.0; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-13C HSQC | sample_3 | isotropic | sample_conditions_3 |
| 2D 1H-13C HSQC | sample_4 | isotropic | sample_conditions_4 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_3 |
| 2D 1H-15N HSQC | sample_4 | isotropic | sample_conditions_4 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
SPARKY, Goddard - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz