BMRB Entry 16414

Title:
Interaction of the Second Binding Region Troponin I with the Regulatory Domain of Skeletal Muscle Troponin C as Determined by NMR Spectroscopy
Deposition date:
2009-07-16
Original release date:
2010-03-05
Authors:
McKay, Ryan; Tripet, Brian; Hodges, Robert; Sykes, Brian
Citation:

Citation: McKay, Ryan; Tripet, Brian; Hodges, Robert; Sykes, Brian. "Interaction of the Second Binding Region of Troponin I with the Regulatory Domain of Skeletal Muscle Troponin C as Determined by NMR Spectroscopy"  J. Biol. Chem. 272, 28494-28500 (1997).
PubMed: 9353310

Assembly members:

Assembly members:
TnC, polymer, 90 residues, Formula weight is not available
TnI, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: Gallus gallus

Data sets:
  • binding_constants
Data typeCount
binding constants2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TnC1
2TnI2

Entities:

Entity 1, TnC 90 residues - Formula weight is not available

1   ALASERMETTHRASPGLNGLNALAGLUALA
2   ARGALAPHELEUSERGLUGLUMETILEALA
3   GLUPHELYSALAALAPHEASPMETPHEASP
4   ALAASPGLYGLYGLYASPILESERTHRLYS
5   GLULEUGLYTHRVALMETARGMETLEUGLY
6   GLNASNPROTHRLYSCYSGLULEUASPALA
7   ILEILECYSGLUVALASPGLUASPGLYSER
8   GLYTHRILEASPPHEGLUGLUPHELEUVAL
9   METMETVALARGGLNMETLYSGLUASPALA

Entity 2, TnI 17 residues - Formula weight is not available

1   ARGMETSERALAASPALAMETLEUARGALA
2   LEULEUGLYSERLYSHISLYS

Samples:

sample_1: TnC, [U-50% 15N; 2H], 1.36 ± 0.03 mM; imidazole 10 mM; DSS 0.1 mM; potassium chloride 0 mM; calcium chloride 5.4 mM; TnI 1.9 mM; H2O 90%; D2O 10%

sample_2: TnC, [U-50% 15N; 2H], 1.54 ± 0.1 mM; imidazole 10 mM; DSS 0.1 mM; potassium chloride 100 mM; calcium chloride 6 mM; TnI 2.72 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.85; pressure: 1 atm; temperature: 304 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP, Garrett - data analysis

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

BMRB 4232 4401 5738
PDB
REF XP_005057071 XP_009321443 XP_009582608 XP_009992324 XP_013053940 NP_001011110 NP_001079556 NP_990748 XP_001518819 XP_002199061
EMBL CAA27447
GB AAA61952 AAB00122 AAH44282 AAH84508 AAL86905
SP P68246 P68247
AlphaFold P68246 P68247