BMRB Entry 16419

Title:
Local Perturbations by Ligand Binding of Hydrogen Deuterium Exchange Kinetics in a Four-helix Bundle Protein, Acyl Coenzyme A Binding Protein (ACBP)
Deposition date:
2009-07-23
Original release date:
2012-06-06
Authors:
Kragelund, Birthe; Knudsen, Jens; Poulsen, Flemming
Citation:

Citation: Kragelund, B.; Knudsen, J.; Poulsen, F.. "Local perturbations by ligand binding of hydrogen deuterium exchange kinetics in a four-helix bundle protein, acyl coenzyme A binding protein (ACBP)."  J. Mol. Biol. 250, 695-706 (1995).
PubMed: 7623386

Assembly members:

Assembly members:
ACBP, polymer, 86 residues, 10043.79 Da.
TH3, non-polymer, 1003.927 Da.

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Data sets:
Data typeCount
chemical rates56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ACBP1
2palmitoyl-CoA2

Entities:

Entity 1, ACBP 86 residues - 10043.79 Da.

1   SERGLNALAGLUPHEASPLYSALAALAGLU
2   GLUVALLYSHISLEULYSTHRLYSPROALA
3   ASPGLUGLUMETLEUPHEILETYRSERHIS
4   TYRLYSGLNALATHRVALGLYASPILEASN
5   THRGLUARGPROGLYMETLEUASPPHELYS
6   GLYLYSALALYSTRPASPALATRPASNGLU
7   LEULYSGLYTHRSERLYSGLUASPALAMET
8   LYSALATYRILEASPLYSVALGLUGLULEU
9   LYSLYSLYSTYRGLYILE

Entity 2, palmitoyl-CoA - C37 H64 N7 O17 P3 S - 1003.927 Da.

1   TH3

Samples:

sample_1: ACBP 2 mM; potassium phosphate 0.04 mM; palmitoyl-CoA 2 mM; D2O 100%

sample_conditions_1: pH: 6.65; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

PRONTO, Kjur, Andersen and Poulsen - data analysis, processing

MNMR - processing

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 2049 2050
PDB
EMBL CAA44618
GB AAA30495 AAI14182 AIM41257 ELR45212
REF NP_001106792 XP_004004799 XP_005910864 XP_006040065 XP_006057227
SP P07107
TPG DAA32604
AlphaFold P07107