BMRB Entry 16580

Title:
2J coupling constants in Ribonuclease T1 from Aspergillus oryzae
Deposition date:
2009-10-25
Original release date:
2010-02-08
Authors:
Schmidt, Jurgen; Lohr, Frank
Citation:

Citation: Schmidt, Jurgen; Hua, Yixun; Lohr, Frank. "Correlation of (2)J couplings with protein secondary structure."  Proteins 78, 1544-1562 (2010).
PubMed: 20131375

Assembly members:

Assembly members:
RNase_T1, polymer, 104 residues, 11089.3 Da.

Natural source:

Natural source:   Common Name: Aspergillus oryzae   Taxonomy ID: 5062   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Aspergillus oryzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pA2T1

Data typeCount
coupling constants824

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1

Entities:

Entity 1, protein 104 residues - 11089.3 Da.

1   ALACYSASPTYRTHRCYSGLYSERASNCYS
2   TYRSERSERSERASPVALSERTHRALAGLN
3   ALAALAGLYTYRLYSLEUHISGLUASPGLY
4   GLUTHRVALGLYSERASNSERTYRPROHIS
5   LYSTYRASNASNTYRGLUGLYPHEASPPHE
6   SERVALSERSERPROTYRTYRGLUTRPPRO
7   ILELEUSERSERGLYASPVALTYRSERGLY
8   GLYSERPROGLYALAASPARGVALVALPHE
9   ASNGLUASNASNGLNLEUALAGLYVALILE
10   THRHISTHRGLYALASERGLYASNASNPHE
11   VALGLUCYSTHR

Samples:

sample_1: RNase_T1, [U-95% 13C; U-95% 15N], 2 mM; D2O, [U-2H], 10%; H2O 90%

sample_2: RNase_T1, [U-95% 13C; U-95% 15N], 2 mM; D2O, [U-2H], 100%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D Ca-coupled [15N,1H]-TROSY-HNCOsample_1isotropicsample_conditions_1
2D IPAP-type HN(CO-a/b-NCa-J)-TROSYsample_1isotropicsample_conditions_1
2D IPAP-type HN(a/b-NCO-J)-TROSYsample_1isotropicsample_conditions_1
3D Ha-coupled ct-[15N,1H]-TROSY-iHNCAsample_1isotropicsample_conditions_1
3D Cb-coupled [15N,1H]-TROSY-HNCAsample_1isotropicsample_conditions_1
3D Ha-coupled [15N,1H]-TROSY-H(N)COCAsample_1isotropicsample_conditions_1
3D Cb-coupled [15N,1H]-TROSY-H(N)COCAsample_1isotropicsample_conditions_1
2D Cb-coupled [13C,1H]-HSQCsample_2isotropicsample_conditions_1
2D CO-coupled ct-[13C,1H]-HSQCsample_2isotropicsample_conditions_1
3D CO-coupled ct-[15N,1H]-TROSY-HNCAsample_1isotropicsample_conditions_1
3D CO-coupled (H)CANNHsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection, processing

jeval, JM Schmidt - coupling constant extraction, data analysis, multiplet simulation

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz
  • Bruker Avance 950 MHz

Related Database Links:

BMRB 15905 16469 1658
PDB
DBJ BAA05707 BAA08407 BAE64671
EMBL CAA30560
GB AAA72541 AAA72994 EED52238 EIT74451 KDE77875
REF XP_001825804 XP_002377402
SP P00651
AlphaFold P00651