BMRB Entry 16677

Title:
Inter-molecular interactions in a 44 kDa interferon-receptor complex detected by asymmetric back-protonation and 2D NOESY
Deposition date:
2010-01-14
Original release date:
2010-05-26
Authors:
Nudelman, Ilona; Akabayov, Sabine; Schnur, Einat; Biron, Zohar; Levy, Rina; Xu, Yingqi; Yang, Daiwen; Anglister, Jacob
Citation:

Citation: Nudelman, Ilona; Akabayov, Sabine; Schnur, Einat; Biron, Zohar; Levy, Rina; Xu, Yingqi; Yang, Daiwen; Anglister, Jacob. "Intermolecular interactions in a 44 kDa interferon-receptor complex detected by asymmetric reverse-protonation and two-dimensional NOESY."  Biochemistry 49, 5117-5133 (2010).
PubMed: 20496919

Assembly members:

Assembly members:
entity_1, polymer, 165 residues, 19264.242 Da.
entity_2, polymer, 212 residues, 24323.609 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTZT7U18

Data sets:
Data typeCount
15N chemical shifts285
1H chemical shifts1665

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 165 residues - 19264.242 Da.

1   CYSASPLEUPROGLNTHRHISSERLEUGLY
2   SERARGARGTHRLEUMETLEULEUALAGLN
3   METARGLYSILESERLEUPHESERCYSLEU
4   LYSASPARGHISASPPHEGLYPHEPROGLN
5   GLUGLUPHEGLYASNGLNPHEGLNLYSALA
6   GLUTHRILEPROVALLEUHISGLUMETILE
7   GLNGLNILEPHEASNLEUPHESERTHRLYS
8   ASPSERSERALAALATRPASPGLUTHRLEU
9   LEUASPLYSPHETYRTHRGLULEUTYRGLN
10   GLNLEUASNASPLEUGLUALACYSVALILE
11   GLNGLYVALGLYVALTHRGLUTHRPROLEU
12   METLYSGLUASPSERILELEUALAVALARG
13   LYSTYRPHEGLNARGILETHRLEUTYRLEU
14   LYSGLULYSLYSTYRSERPROCYSALATRP
15   GLUVALVALARGALAGLUILEMETARGSER
16   PHESERLEUSERTHRASNLEUGLNGLUSER
17   LEUARGSERLYSGLU

Entity 2, entity_2 212 residues - 24323.609 Da.

1   SERTYRASPSERPROASPTYRTHRASPGLU
2   SERCYSTHRPHELYSILESERLEUARGASN
3   PHEARGSERILELEUSERTRPGLULEULYS
4   ASNHISSERILEVALPROTHRHISTYRTHR
5   LEULEUTYRTHRILEMETSERLYSPROGLU
6   ASPLEULYSVALVALLYSASNCYSALAASN
7   THRTHRARGSERPHECYSASPLEUTHRASP
8   GLUTRPARGSERTHRHISGLUALATYRVAL
9   THRVALLEUGLUGLYPHESERGLYASNTHR
10   THRLEUPHESERCYSSERHISASNPHETRP
11   LEUALAILEASPMETSERPHEGLUPROPRO
12   GLUPHEGLUILEVALGLYPHETHRASNHIS
13   ILEASNVALMETVALLYSPHEPROSERILE
14   VALGLUGLUGLULEUGLNPHEASPLEUSER
15   LEUVALILEGLUGLUGLNSERGLUGLYILE
16   VALLYSLYSHISLYSPROGLUILELYSGLY
17   ASNMETSERGLYASNPHETHRTYRILEILE
18   ASPLYSLEUILEPROASNTHRASNTYRCYS
19   VALSERVALTYRLEUGLUHISSERASPGLU
20   GLNALAVALILELYSSERPROLEULYSCYS
21   THRLEULEUPROPROGLYGLNGLUSERGLU
22   PHESER

Samples:

sample_1: entity_1, [U-2H; NA-H,F,W,Y], 0.25 mM; entity_2, [U-2H; NA-V,L,T,M,A,K], 0.25 mM; TRIS, [U-2H], 25 mM; sodium azide 0.02%; D2O 100%

sample_2: entity_1, [U-2H; NA-K,R,L,A,M], 0.25 mM; entity_2, [U-2H; NA-H,F,W], 0.25 mM; TRIS, [U-2H], 25 mM; sodium azide 0.02%; D2O 100%

sample_3: entity_1, [U-2H; NA-L], 0.25 mM; entity_2, [U-2H; NA-H,W], 0.25 mM; TRIS, [U-2H], 25 mM; sodium azide 0.02%; D2O 100%

sample_4: entity_1 0.25 mM; entity_2, [U-15N], 0.25 mM; TRIS, [U-2H], 25 mM; sodium azide 0.02%; D2O 100%

sample_5: entity_1, [U-15N], 0.25 mM; entity_2 0.25 mM; TRIS, [U-2H], 25 mM; sodium azide 0.02%; D2O 100%

sample_conditions_1: ionic strength: 0 M; pH: 8.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1
15N-separated NOESYsample_4isotropicsample_conditions_1
15N-separated NOESYsample_5isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 18135 18344 4081 5049
PDB
DBJ BAJ20902 BAF85748 BAI46204
EMBL CAA23805 CAA23809 CAA23810 CAA25770 CAA46954 CAA54785 CAA61914 CAA61940 CAG46616 CDG37809
GB AAA52715 AAA59181 AAB59402 AAC64915 AAH74936 AAB46413 AAB46414 AAB46415 AAB46417 AAB46418
PRF 0611561A 0906342A
REF NP_000596 XP_003830724 XP_004047919 XP_528568 NP_000865 NP_001276054 NP_001276055 NP_001276057 NP_997467
SP P01563 P48551
AlphaFold P01563 P48551

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks