BMRB Entry 16905

Title:
Sma0114
Deposition date:
2010-04-29
Original release date:
2010-10-27
Authors:
Sheftic, Sarah; Gage, Dan; Alexandrescu, Andrei
Citation:

Citation: Sheftic, Sarah; Garcia, Preston; Robinson, Victoria; Gage, Daniel; Alexandrescu, Andrei. "NMR assignments for the Sinorhizobium meliloti response regulator Sma0114."  Biomol. NMR Assignments 5, 55-58 (2011).
PubMed: 20936511

Assembly members:

Assembly members:
Sma0114, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Sinorhizobium meliloti   Taxonomy ID: 382   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Sinorhizobium meliloti

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28(+)

Data sets:
Data typeCount
13C chemical shifts478
15N chemical shifts119
1H chemical shifts660
coupling constants78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1response regulator1

Entities:

Entity 1, response regulator 123 residues - Formula weight is not available

1   GLYSERHISMETTHRGLUARGARGLEUARG
2   VALLEUVALVALGLUASPGLUSERMETILE
3   ALAMETLEUILEGLUASPTHRLEUCYSGLU
4   LEUGLYHISGLUVALALAALATHRALASER
5   ARGMETGLNGLUALALEUASPILEALAARG
6   LYSGLYGLNPHEASPILEALAILEILEASP
7   VALASNLEUASPGLYGLUPROSERTYRPRO
8   VALALAASPILELEUALAGLUARGASNVAL
9   PROPHEILEPHEALATHRGLYTYRGLYSER
10   LYSGLYLEUASPTHRARGTYRSERASNILE
11   PROLEULEUTHRLYSPROPHELEUASPSER
12   GLULEUGLUALAVALLEUVALGLNILESER
13   LYSGLUVAL

Samples:

sample_1: Sma114, [U-100% 15N], 500 uM; H2O 90%; D2O 10%; sodium phosphate 50 mM; DTT 1 mM

sample_2: Sma114, [U-100% 13C; U-100% 15N], 500 uM; D2O 100%; sodium phosphate 50 mM; DTT 1 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
HSQC TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HNHBsample_2isotropicsample_conditions_1
3D HNHAsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 19286
PDB
EMBL CCM71308 CDH82688
GB AAK64716 AEG06710 AEG57042 AEH82764 AGG69741
REF NP_435304 WP_010967059 WP_033048070 WP_046066705

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks