BMRB Entry 17096

Title:
Stopped-flow NMR spectroscopy: Real-Time unfolding studies of 6-19F-tryptophan-labeled Escherichia coli dihydrofolate reductase
Deposition date:
2010-08-02
Original release date:
2010-11-10
Authors:
Hoeltzli, Sydney; Frieden, Carl
Citation:

Citation: Hoeltzli, Sydney; Frieden, Carl. "Stopped-flow NMR spectroscopy: Real-time unfolding studies of 6-19F-tryptophan-labeled Escherichia coli dihydrofolate reductase"  Proc. Natl. Acad. Sci. U.S.A. 92, 9318-9322 (1995).
PubMed: 7568125

Assembly members:

Assembly members:
DHFR, polymer, 187 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMONDHFR

Data sets:
Data typeCount
kinetic rates1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DHFR1

Entities:

Entity 1, DHFR 187 residues - Formula weight is not available

1   METVALGLYSERLEUASNCYSILEVALALA
2   VALSERGLNASNMETGLYILEGLYLYSASN
3   GLYASPLEUPROTRPPROPROLEUARGASN
4   GLUPHEARGTYRPHEGLNARGMETTHRTHR
5   THRSERSERVALGLUGLYLYSGLNASNLEU
6   VALILEMETGLYLYSLYSTHRTRPPHESER
7   ILEPROGLULYSASNARGPROLEULYSGLY
8   ARGILEASNLEUVALLEUSERARGGLULEU
9   LYSGLUPROPROGLNGLYALAHISPHELEU
10   SERARGSERLEUASPASPALALEULYSLEU
11   THRGLUGLNPROGLULEUALAASNLYSVAL
12   ASPMETVALTRPILEVALGLYGLYSERSER
13   VALTYRLYSGLUALAMETASNHISPROGLY
14   HISLEULYSLEUPHEVALTHRARGILEMET
15   GLNASPPHEGLUSERASPTHRPHEPHEPRO
16   GLUILEASPLEUGLULYSTYRLYSLEULEU
17   PROGLUTYRPROGLYVALLEUSERASPVAL
18   GLNGLUGLULYSGLYILELYSTYRLYSPHE
19   GLUVALTYRGLULYSASNASP

Samples:

sample_1: potassium phosphate 50 mM; EDTA 0.1 mM; DTT 3 mM; sodium azide 5 mM; DHFR, [U-100% 19F-tryptophan], 0.89 mM; urea0 – 5 M; H2O 85%; D2O 15%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
19F NMRsample_1isotropicsample_conditions_1

Software:

KaleidaGraph, Synergy Software, Reading, PA - exponential fits

VNMR, Varian - data analysis

NMR spectrometers:

  • Varian VXR-500 470.3 MHz

Related Database Links:

BMRB 19563 19564 19565 19566 19567
PDB
DBJ BAG35526 BAG56693 BAG59770 BAJ20267
EMBL CAA23765 CAA25409
GB AAA58484 AAA58485 AAH00192 AAH03584 AAH70280
PRF 0905195A 0906214A
REF NP_000782 NP_001277283 NP_001277286 XP_001099963 XP_001110551
SP P00374
AlphaFold P00374