BMRB Entry 17114

Title:
Binding Kinetics of Histone Chaperone Chz1 and Variant Histone H2A.Z-H2B by Relaxation Dispersion NMR Spectroscopy
Deposition date:
2010-08-11
Original release date:
2010-11-10
Authors:
Hansen, D.; Zhou, Zheng; Feng, Haniqiao; Jenkins, Lisa; Bai, Yawen; Kay, Lewis
Citation:

Citation: Hansen, D.; Zhou, Zheng; Feng, Haniqiao; Jenkins, Lisa; Bai, Yawen; Kay, Lewis. "Binding Kinetics of Histone Chaperone Chz1 and Variant Histone H2A.Z-H2B by Relaxation Dispersion NMR Spectroscopy"  J. Mol. Biol. 387, 1-9 (2009).
PubMed: 19385041

Assembly members:

Assembly members:
H2AZH2B, polymer, 192 residues, Formula weight is not available
Chz1, polymer, 62 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Data sets:
  • chemical rates
Data typeCount
binding constants2
kinetic rates3

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H2AZH2B1
2Chz12

Entities:

Entity 1, H2AZH2B 192 residues - Formula weight is not available

1   ARGLYSGLUTHRTYRSERSERTYRILETYR
2   LYSVALLEULYSGLNTHRHISPROASPTHR
3   GLYILESERGLNLYSSERMETSERILELEU
4   ASNSERPHEVALASNASPILEPHEGLUARG
5   ILEALATHRGLUALASERLYSLEUALAALA
6   TYRASNLYSLYSSERTHRILESERALAARG
7   GLUILEGLNTHRALAVALARGLEUILELEU
8   PROGLYGLULEUALALYSHISALAVALSER
9   GLUGLYTHRARGALAVALTHRLYSTYRSER
10   SERSERTHRGLNALAGLNSERSERSERALA
11   ARGALAGLYLEUGLNPHEPROVALGLYARG
12   ILELYSARGTYRLEULYSARGHISALATHR
13   GLYARGTHRARGVALGLYSERLYSALAALA
14   ILETYRLEUTHRALAVALLEUGLUTYRLEU
15   THRALAGLUVALLEUGLULEUALAGLYASN
16   ALAALALYSASPLEULYSVALLYSARGILE
17   THRPROARGHISLEUGLNLEUALAILEARG
18   GLYASPASPGLULEUASPSERLEUILEARG
19   ALATHRILEALASERGLYGLYVALLEUPRO
20   HISILE

Entity 2, Chz1 62 residues - Formula weight is not available

1   THRVALGLUASPSERGLUSERASPMETASP
2   ASPALALYSLEUASPALALEUMETGLYASN
3   GLUGLYGLUGLUGLUGLUASPASPLEUALA
4   GLUILEASPTHRSERASNILEILETHRSER
5   GLYARGARGTHRARGGLYLYSVALILEASP
6   TYRLYSLYSTHRALAGLUGLULEUASPLYS
7   LYSGLU

Samples:

sample_1: H2AZH2B, [U-15N; U-2H], 0.95 mM; Chz1, [U-15N; U-2H], 1 mM; Mes 25 mM; sodium chloride 0.2 M; EDTA 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.0; pressure: ambient atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

FuDA, flemming@pound.med.utoronto.ca; http://pound.med.utoronto.ca/software - signal quantification

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 15393 15393
PDB
EMBL CAY78724 CAY79198
GB AJV19600 AAB64563 AHY75583 EDN62999 EDV08859 EDZ72631
DBJ GAA22856
REF NP_010947
SP A6ZQX9 P40019
TPG DAA07683
AlphaFold A6ZQX9 P40019