BMRB Entry 17121

Title:
Folding Kinetics of the SH3 Domain of PI3 Kinase by Real-time NMR Combined with Optical Spectroscopy
Deposition date:
2010-08-11
Original release date:
2010-11-10
Authors:
Guijarro, J.; Morton, Craig; Plaxco, Kevin; Campbell, Iain; Dobson, Christopher
Citation:

Citation: Guijarro, J.; Morton, Craig; Plaxco, Kevin; Campbell, Iain; Dobson, Christopher. "Folding Kinetics of the SH3 Domain of PI3 Kinase by Real-time NMR Combined with Optical Spectroscopy"  J. Mol. Biol. 276, 657-667 (1998).
PubMed: 9551103

Assembly members:

Assembly members:
PISH3, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Data typeCount
kinetic rates4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PISH31

Entities:

Entity 1, PISH3 90 residues - Formula weight is not available

84 residues from PI3-SH3 domain + 2 residue N terminal addition (GS) and 4 residue C terminal addition (WNSS)

1   GLYSERMETSERALAGLUGLYTYRGLNTYR
2   ARGALALEUTYRASPTYRLYSLYSGLUARG
3   GLUGLUASPILEASPLEUHISLEUGLYASP
4   ILELEUTHRVALASNLYSGLYSERLEUVAL
5   ALALEUGLYPHESERASPGLYGLNGLUALA
6   ARGPROGLUGLUILEGLYTRPLEUASNGLY
7   TYRASNGLUTHRTHRGLYGLUARGGLYASP
8   PHEPROGLYTHRTYRVALGLUTYRILEGLY
9   ARGLYSLYSILESERPROTRPASNSERSER

Samples:

sample_1: PISH3, [U-2H], 10 mg/ml; NaH2PO4 20 mM; guanidine hydrochloride, [U-2H], .727 M; D2O 100%

sample_conditions_1: pH: 7.2; pressure: ambient atm; temperature: 279 K

sample_conditions_2: pH: 7.2; pressure: ambient atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H NMRsample_1isotropicsample_conditions_1
1H NMRsample_1isotropicsample_conditions_2

Software:

FELIX v2.3, Accelrys Software Inc. - data analysis

KaleidaGraph, Synergy Software - data analysis

NMR spectrometers:

  • Home Built na 600 MHz

Related Database Links:

BMRB 16448 19110
PDB
DBJ BAA18932
EMBL CAH92731
GB AAA79511 AAC52847 AAH26146 AAH94795 ABK42316
PRF 2210313A
REF NP_001070963 NP_001126593 NP_001248126 NP_037137 NP_777000
SP P23727 P26450 P27986 Q5R685 Q63787
TPG DAA17994
AlphaFold Q63787 P23727 P26450 P27986 Q5R685