BMRB Entry 17340

Title:
An Antibody Binding Site on Cytochrome c Defined by Hydrogen Exchange and Two-Dimensional NMR
Deposition date:
2010-12-02
Original release date:
2016-06-09
Authors:
Paterson, Yvonne; Englander, S.; Roder, Heinrich
Citation:

Citation: Paterson, Yvonne; Englander, S.; Roder, Heinrich. "An Antibody Binding Site on Cytochrome c Defined by Hydrogen Exchange and Two-Dimensional NMR"  Science 249, 755-759 (1990).
PubMed: 1697101

Assembly members:

Assembly members:
cytc, polymer, 104 residues, Formula weight is not available
MABE8, polymer, 219 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Horse   Taxonomy ID: 9796   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:

Experimental source:   Production method: purified from the natural source

Data sets:
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytc1
2MABE82

Entities:

Entity 1, cytc 104 residues - Formula weight is not available

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   VALGLNLYSCYSALAGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYPHETHRTYRTHRASP
6   ALAASNLYSASNLYSGLYILETHRTRPLYS
7   GLUGLUTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEALAGLYILELYSLYSLYSTHRGLU
10   ARGGLUASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, MABE8 219 residues - Formula weight is not available

1   GLUVALGLNLEUGLNGLNSERGLYALAGLU
2   LEUVALLYSPROGLYALASERVALLYSLEU
3   SERCYSTHRALASERGLYPHEASNILELYS
4   ASPTHRTYRMETHISTRPVALLYSGLNARG
5   PROGLULYSGLYLEUGLUTRPILEGLYARG
6   ILEASPPROALASERGLYASNTHRLYSTYR
7   ASPPROLYSPHEGLNASPLYSALATHRILE
8   THRALAASPTHRSERSERASNTHRALATYR
9   LEUGLNLEUSERSERLEUTHRSERGLUASP
10   THRALAVALTYRTYRCYSALAGLYTYRASP
11   TYRGLYASNPHEASPTYRTRPGLYGLNGLY
12   THRTHRLEUTHRVALSERSERALAGLUTHR
13   THRPROPROSERVALTYRPROLEUALAPRO
14   GLYTHRALAALALEULYSSERSERMETVAL
15   THRLEUGLYCYSLEUVALLYSGLYTYRPHE
16   PROGLUPROVALTHRVALTHRTRPASNSER
17   GLYSERLEUSERSERGLYVALHISTHRPHE
18   PROALAVALLEUGLNSERASPLEUTYRTHR
19   LEUSERSERSERVALTHRVALPROSERSER
20   THRTRPPROSERGLNTHRVALTHRCYSASN
21   VALALAHISPROALASERSERTHRLYSVAL
22   ASPLYSLYSILEVALPROARGASNCYS

Samples:

sample_1: cytc 1.5 mM; MABE80 – 1.5 mM; potassium phosphate .1 M; ascorbic acid 9 mM; D2O 100%

sample_conditions_1: pH: 5.3; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D NMRsample_1isotropicsample_conditions_1

Software:

FTNMR, Hare Research, Woodinville, Washington - processing

ModelFree -

NMR spectrometers:

  • Bruker AM 500 MHz

Related Database Links:

PDB