BMRB Entry 18007

Title:
TBA
Deposition date:
2011-10-18
Original release date:
2011-11-02
Authors:
Tripsianes, Konstantinos; Madl, T.; Machyna, M.; Fessas, D.; Englbrecht, C.; Neugebauer, U.; Sattler, M.
Citation:

Citation: Tripsianes, Konstantinos; Madl, T.; Machyna, M.; Fessas, D.; Englbrecht, C.; Fischer, U.; Neugebauer, K.; Sattler, M.. "Structural basis for dimethylarginine recognition by the Tudor domains of human SMN and SPF30 proteins"  Nat. Struct. Mol. Biol. 18, 1414-1420 (2011).
PubMed: 22101937

Assembly members:

Assembly members:
A, polymer, 64 residues, 7084.873 Da.
DA2, non-polymer, 202.254 Da.

Natural source:

Natural source:   Common Name: HUMAN   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: HOMO SAPIENS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: na

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts212
15N chemical shifts68
1H chemical shifts448

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2NG,NG-DIMETHYL-L-ARGININE2

Entities:

Entity 1, protein 64 residues - 7084.873 Da.

1   ASNTHRALAALASERLEUGLNGLNTRPLYS
2   VALGLYASPLYSCYSSERALAILETRPSER
3   GLUASPGLYCYSILETYRPROALATHRILE
4   ALASERILEASPPHELYSARGGLUTHRCYS
5   VALVALVALTYRTHRGLYTYRGLYASNARG
6   GLUGLUGLNASNLEUSERASPLEULEUSER
7   PROILECYSGLU

Entity 2, NG,NG-DIMETHYL-L-ARGININE - C8 H18 N4 O2 - 202.254 Da.

1   DA2

Samples:

sample_new_1: SMN, [U-13C; U-15N], 4 mM; DA2 18 mM; H2O 93%; D2O 7%; phosphate 20 mM; NaCl 50 mM

sample_conditions_new_1: ionic strength: 50.000 mM; pH: 6.5; pressure: 1.000 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-EDITED 3D NOESY F1 15N/13C FILTEREDsample_new_1solutionsample_conditions_new_1
15N-EDITED 3D NOESYsample_new_1solutionsample_conditions_new_1
13C-EDITED 3D NOESY (ALIPHATICSS) F1 15N/13C FILTEREDsample_new_1solutionsample_conditions_new_1
13C-EDITED 3D NOESY (ALIPHATICS)sample_new_1solutionsample_conditions_new_1
13C-EDITED 3D NOESY (AROMATICS) F1 15N/13C FILTEREDsample_new_1solutionsample_conditions_new_1
13C-EDITED 3D NOESY (AROMATICS)sample_new_1solutionsample_conditions_new_1

Software:

AutoDep v4.3, AutoDep - chemical shift assignment

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

ANALYSIS v2.1, CCPN - chemical shift assignment

TALOS vany, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • BRUKER AVANCE 750 MHz

Related Database Links:

UNP SMN_HUMAN
BMRB 18005
PDB
DBJ BAF82358 BAJ21116
EMBL CAH89989
GB AAA64505 AAA66242 AAC50473 AAC52048 AAC62262
PRF 2208336A
REF NP_000335 NP_001124942 NP_001284644 NP_059107 NP_075012
SP Q16637 Q5RE18
AlphaFold Q96J51 Q16637 Q5RE18

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks