BMRB Entry 20088

Title:
Novel Helix-Constrained Nociceptin Derivatives Are Potent Agonists and Antagonists of ERK Phosphorylation and Thermal Analgesia in Mice
Deposition date:
2009-07-23
Original release date:
2011-06-03
Authors:
Fairlie, David; Ruiz-Gomez, Gloria
Citation:

Citation: Harrison, Rosemary; Ruiz-Gomez, Gloria; Hill, Timothy; Chow, Shiao; Shepherd, Nicholas; Lohman, Rink-Jan; Abbenante, Giovanni; Hoang, Huy; Fairlie, David. "Novel Helix-Constrained Nociceptin Derivatives Are Potent Agonists and Antagonists of ERK Phosphorylation and Thermal Analgesia in Mice."  J. Med. Chem. 53, 8400-8408 (2010).
PubMed: 21067234

Assembly members:

Assembly members:
nociceptin_analogue, polymer, 19 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo Sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
nociceptin_analogue: FGGXTGKRKSDRKKKNQDX

Data sets:
Data typeCount
1H chemical shifts127
coupling constants17

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nociceptin analogue1

Entities:

Entity 1, nociceptin analogue 19 residues - Formula weight is not available

1   PHEGLYGLYPFFTHRGLYLYSARGLYSSER
2   ASPARGLYSLYSLYSASNGLNASPNH2

Samples:

sample_1: nociceptin analogue2 – 5 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 4.5; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 20087 20089