BMRB Entry 25337

Title:
Backbone assignments for human Cyclophilin A with saturating concentrations of the model peptide GSFGPDLRAGD
Deposition date:
2014-11-13
Original release date:
2014-12-16
Authors:
Holliday, Michael; Camilloni, Carlo; Armstrong, Geoffrey; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan
Citation:

Citation: Holliday, Michael; Camilloni, Carlo; Armstrong, Geoffrey; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan. "Structure and Dynamics of GeoCyp: A Thermophilic Cyclophilin with a Novel Substrate Binding Mechanism That Functions Efficiently at Low Temperatures"  Biochemistry 54, 3207-3217 (2015).
PubMed: 25923019

Assembly members:

Assembly members:
CypA, polymer, 165 residues, 18017.4843 Da.
GSFGPDLRAGD, polymer, 11 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts157
1H chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CypA1
2GSFGPDLRAGD2

Entities:

Entity 1, CypA 165 residues - 18017.4843 Da.

Full length human Cyclophilin A

1   METVALASNPROTHRVALPHEPHEASPILE
2   ALAVALASPGLYGLUPROLEUGLYARGVAL
3   SERPHEGLULEUPHEALAASPLYSVALPRO
4   LYSTHRALAGLUASNPHEARGALALEUSER
5   THRGLYGLULYSGLYPHEGLYTYRLYSGLY
6   SERCYSPHEHISARGILEILEPROGLYPHE
7   METCYSGLNGLYGLYASPPHETHRARGHIS
8   ASNGLYTHRGLYGLYLYSSERILETYRGLY
9   GLULYSPHEGLUASPGLUASNPHEILELEU
10   LYSHISTHRGLYPROGLYILELEUSERMET
11   ALAASNALAGLYPROASNTHRASNGLYSER
12   GLNPHEPHEILECYSTHRALALYSTHRGLU
13   TRPLEUASPGLYLYSHISVALVALPHEGLY
14   LYSVALLYSGLUGLYMETASNILEVALGLU
15   ALAMETGLUARGPHEGLYSERARGASNGLY
16   LYSTHRSERLYSLYSILETHRILEALAASP
17   CYSGLYGLNLEUGLU

Entity 2, GSFGPDLRAGD 11 residues - Formula weight is not available

1   GLYSERPHEGLYPROASPLEUARGALAGLY
2   ASP

Samples:

CypA-GSFGPDLRAGD: CypA, [U-100% 13C; U-100% 15N], 1 mM; GSFGPDLRAGD 4 mM; sodium phosphate 50 mM; EDTA 1 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBCypA-GSFGPDLRAGDisotropicsample_conditions
3D CBCA(CO)NHCypA-GSFGPDLRAGDisotropicsample_conditions
2D 1H-15N HSQC/HMQCCypA-GSFGPDLRAGDisotropicsample_conditions
3D HNCACypA-GSFGPDLRAGDisotropicsample_conditions

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment, peak picking

nmrPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Varian - Spectrum processing, collection

NMR spectrometers:

  • Varian UnityInova 800 MHz
  • Varian UnityInova 600 MHz

Related Database Links:

BMRB 17218 2208 25104 25502
PDB
DBJ BAE01146 BAE30323 BAE87660 BAF82774 BAF83540
EMBL CAA34961 CAA37039 CAA68264 CAG32988 CAH91833
GB AAB81959 AAB81960 AAB81961 AAF69142 AAF78600
PIR CSHYAC
PRF 1503232A
REF NP_001009370 NP_001027981 NP_001126060 NP_001270275 NP_001271703
SP P14851 P62935 P62936 P62937 P62938
TPG DAA25853 DAA25854
AlphaFold P62936 P62937 P62938 P14851 P62935

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks