BMRB Entry 4130

Title:
1H, 15N, 13C Chemical Shifts of Recombinant Rat Ferrocytochrome b5, A conformation
Deposition date:
1998-04-07
Original release date:
1998-08-12
Authors:
Dangi, Bindi; Sarma, Siddhartha; Yan, Chunhua; Banville, Debra; Guiles, Ronald
Citation:

Citation: Dangi, Bindi; Sarma, Siddhartha; Yan, Chunhua; Banville, Debra; Guiles, Ronald. "The Origin of Difference in the Physical Properties of the Equilibrium Forms of Cytochrome b5 Revealed Through High Resolution NMR Structures and Backbone Dynamic Analyses"  Biochemistry 37, 8289-8302 (1998).

Assembly members:

Assembly members:
cytochrome b5, polymer, 94 residues, Formula weight is not available
HEME C, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: rat   Taxonomy ID: 10117   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus rattus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3c

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts514
15N chemical shifts90
13C chemical shifts297

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytb5_A1
2heme2

Entities:

Entity 1, cytb5_A 94 residues - Formula weight is not available

1   ASPLYSASPVALLYSTYRTYRTHRLEUGLU
2   GLUILEGLNLYSHISLYSASPSERLYSSER
3   THRTRPVALILELEUHISHISLYSVALTYR
4   ASPLEUTHRLYSPHELEUGLUGLUHISPRO
5   GLYGLYGLUGLUVALLEUARGGLUGLNALA
6   GLYGLYASPALATHRGLUASNPHEGLUASP
7   VALGLYHISSERTHRASPALAARGGLULEU
8   SERLYSTHRTYRILEILEGLYGLULEUHIS
9   PROASPASPARGSERLYSILEALALYSPRO
10   SERGLUTHRLEU

Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_one: cytochrome b5, [U-15N; U-13C], 4 mM; phosphate 100 mM

sample_conditions_one: ionic strength: 100 .; pH: 7.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker DMX600 600 MHz

Related Database Links:

BMRB 4131
PDB
DBJ BAA02492 BAB22093 BAB28714 BAE20982
GB AAA67175 AAA72420 AAA72421 AAA72557 AAA99718
REF NP_001268328 NP_071581 NP_080073 XP_003512427 XP_005356388
SP P00173 P56395
AlphaFold P00173 P56395

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks