BMRB Entry 4167

Title:
Backbone H, CA, N chemical shift assignments for C2A/Ca2+/6PS ternary complex
Deposition date:
1998-07-21
Original release date:
2000-03-27
Authors:
Chae, Young; Markley, John
Citation:

Citation: Chae, Young; Markley, John. "Lipid-induced Local Conformational Changes in the C2A-domain of Synaptotagmin I as Revealed by NMR Spectroscopy"  J. Biol. Chem. 273, 25659-25663 (1998).

Assembly members:

Assembly members:
C2A domain of synaptotagmin I, polymer, 128 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.
., non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: black rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts128
1H chemical shifts121
15N chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C2A-SynI1
2entity_CA2
36PS3

Entities:

Entity 1, C2A-SynI 128 residues - Formula weight is not available

1   GLULYSLEUGLYLYSLEUGLNTYRSERLEU
2   ASPTYRASPPHEGLNASNASNGLNLEULEU
3   VALGLYILEILEGLNALAALAGLULEUPRO
4   ALALEUASPMETGLYGLYTHRSERASPPRO
5   TYRVALLYSVALPHELEULEUPROGLULYS
6   LYSLYSLYSPHEGLUTHRLYSVALHISARG
7   LYSTHRLEUASNPROVALPHEASNGLUGLN
8   PHETHRPHELYSVALPROTYRSERGLULEU
9   GLYGLYLYSTHRLEUVALMETALAVALTYR
10   ASPPHEASPARGPHESERLYSHISASPILE
11   ILEGLYGLUPHELYSVALPROMETASNTHR
12   VALASPPHEGLYHISVALTHRGLUGLUTRP
13   ARGASPLEUGLNSERALAGLULYS

Entity 2, entity_CA - Ca - 40.078 Da.

1   CA

Entity 3, 6PS 1 residues - . - Formula weight is not available

1   6PS

Samples:

sample_one: C2A domain of synaptotagmin I, [U-99% 13C; U-99% 15N], 1.0 mM; Calcium 5 mM; . 5 mM; Tris-HCl 50 mM; NaCl 200 mM; DSS 2 mM

sample_conditions_one: pH: 7.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_onenot availablesample_conditions_one
HNCOCAsample_onenot availablesample_conditions_one

Software:

Felix 95 - 1

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 15785 15952 17102 4039 4041 4166
PDB
GB AAK15077 ELW70522 EPY85163 KFO26677
REF XP_005976391

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks