BMRB Entry 4440

Title:
Hyperfine Cys Proton Signals from Reduced Human Ferredoxin
Deposition date:
1999-10-15
Original release date:
2000-01-12
Authors:
Xia, Bin; Jenk, Daniel; LeMaster, David; Westler, William; Markley, John
Citation:

Citation: Xia, Bin; Jenk, Daniel; LeMaster, David; Westler, William; Markley, John. "Electron-Nuclear Interactions in two Prototypical [2Fe-2S] Proteins: Selective (Chiral) Deuteration and Analysis of 1H and 2H NMR Signals from the Alpha and Beta Hydrogens of Cysteinyl Residues that Ligate the Iron in the Active Sites of Human Ferredoxin and Anabaena 7120 Vegetative Ferredoxin"  Arch. Biochem. Biophys. ., .-..

Assembly members:

Assembly members:
Human Ferredoxin, polymer, 124 residues, Formula weight is not available
FE2/S2 (INORGANIC) CLUSTER, non-polymer, 175.820 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts12

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Ferredoxin1
2FES2

Entities:

Entity 1, Human Ferredoxin 124 residues - Formula weight is not available

1   SERSERSERGLUASPLYSILETHRVALHIS
2   PHEILEASNARGASPGLYGLUTHRLEUTHR
3   THRLYSGLYLYSVALGLYASPSERLEULEU
4   ASPVALVALVALGLUASNASNLEUASPILE
5   ASPGLYPHEGLYALACYSGLUGLYTHRLEU
6   ALACYSSERTHRCYSHISLEUILEPHEGLU
7   ASPHISILETYRGLULYSLEUASPALAILE
8   THRASPGLUGLUASNASPMETLEUASPLEU
9   ALATYRGLYLEUTHRASPARGSERARGLEU
10   GLYCYSGLNILECYSLEUTHRLYSSERMET
11   ASPASNMETTHRVALARGVALPROGLUTHR
12   VALALAASPALAARGGLNSERILEASPVAL
13   GLYLYSTHRSER

Entity 2, FES - Fe2 S2 - 175.820 Da.

1   FES

Samples:

HuFd_reduced: Human Ferredoxin2 – 2 mM

sample_conditions: pH*: 7.2; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
not availableHuFd_reducednot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 400 MHz

Related Database Links:

BMRB 4073 4074 4439 5337 6026
GenBank AAA35829 AAA35855 AAA35856 AAA50462 AAA76853
REF NP_004100 XP_001105034 XP_508877
SWISS-PROT P10109