BMRB Entry 4707

Title:
WT1-KTSs free
Deposition date:
2000-03-30
Original release date:
2000-04-28
Authors:
Laity, John; Chung, John; Dyson, H.; Wright, Peter
Citation:

Citation: Laity, John; Chung, John; Dyson, H.; Wright, Peter. "Alternative Splicing of Wilms' Tumor Suppressor Protein Modulates DNA Binding Activity Through Isoform-Specific DNA-Induced Conformational Changes"  Biochemistry ., .-. (2000).

Assembly members:

Assembly members:
WT1, polymer, 119 residues, 14450 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET21

Data sets:
Data typeCount
1H chemical shifts110
13C chemical shifts350
15N chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WT1-KTS1
2Zn 12
3Zn 22
4Zn 32
5Zn 42

Entities:

Entity 1, WT1-KTS 119 residues - 14450 Da.

1   ALASERGLULYSARGPROPHEMETCYSALA
2   TYRPROGLYCYSASNLYSARGTYRPHELYS
3   LEUSERHISLEUGLNMETHISSERARGLYS
4   HISTHRGLYGLULYSPROTYRGLNCYSASP
5   PHELYSASPCYSGLUARGARGPHESERARG
6   SERASPGLNLEULYSARGHISGLNARGARG
7   HISTHRGLYVALLYSPROPHEGLNCYSLYS
8   THRCYSGLNARGLYSPHESERARGSERASP
9   HISLEULYSTHRHISTHRARGTHRHISTHR
10   GLYGLULYSPROPHESERCYSARGTRPPRO
11   SERCYSGLNLYSLYSPHEALAARGSERASP
12   GLULEUVALARGHISHISASNMETHIS

Entity 2, Zn 1 - Zn - 65.409 Da.

1   ZN

Samples:

free_proteins: WT1, [U-95% 13C; U-95% 15N], 1.0 – 1.5 mM

Conditions: pH: 6.70; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCfree_proteinsnot availableConditions
HNCAfree_proteinsnot availableConditions
HN(CO)CAfree_proteinsnot availableConditions
HNCACBfree_proteinsnot availableConditions
CBCA(CO)NHfree_proteinsnot availableConditions
HNCOfree_proteinsnot availableConditions
HCACO(CA)NHfree_proteinsnot availableConditions
HNCACB (deuterium decoupling)free_proteinsnot availableConditions
HN(CO)CACB (deuterium decoupling)free_proteinsnot availableConditions

Software:

FELIX v95, 97 - processing, peakpicking, analysis

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 15532 15533 4708 4709 4710
PDB
DBJ BAA11522 BAA28147 BAA94793 BAF84425
EMBL CAA35956 CAA43819 CDG23662
GB AAA36810 AAA61299 AAB33443 AAB53152 AAH32861
PRF 1604420A
REF NP_000369 NP_001001264 NP_001079057 NP_001079336 NP_001135625
SP B5DE03 O62651 P19544 P79958
TPG DAA21902
AlphaFold B5DE03 O62651 P79958 P19544

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks