BMRB Entry 5000

Title:
1H, 15N and 13C Resonance Assignments for the N-Terminal Domain of the MAPK Phosphatase PAC-1
Deposition date:
2000-04-26
Original release date:
2002-05-09
Authors:
Chaturvedi, Gaurav; Zheng, Lei; Zhou, Ming-Ming
Citation:

Citation: Chaturvedi, Gaurav; Farooq, Amjad; Zheng, Lei; Zhou, Ming-Ming. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments of the Catalytic Domain of the MAPK Phosphatase, PAC-1"  J. Biomol. NMR 25, 79-80 (2003).

Assembly members:

Assembly members:
MAP Kinase Phosphatase PAC-1, polymer, 141 residues, 15100 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts685
13C chemical shifts300
15N chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAC-1 C-Terminal Domain1

Entities:

Entity 1, PAC-1 C-Terminal Domain 141 residues - 15100 Da.

1   VALGLUILELEUPROTYRLEUPHELEUGLY
2   SERCYSSERHISSERSERASPLEUGLNGLY
3   LEUGLNALACYSGLYILETHRALAVALLEU
4   ASNVALSERALASERCYSPROASNHISPHE
5   GLUGLYLEUPHEARGTYRLYSSERILEPRO
6   VALGLUASPASNGLNMETVALGLUILESER
7   ALATRPPHEGLNGLUALAILEGLYPHEILE
8   ASPTRPVALLYSASNSERGLYGLYARGVAL
9   LEUVALHISSERGLNALAGLYILESERARG
10   SERALATHRILECYSLEUALATYRLEUMET
11   GLNSERARGARGVALARGLEUASPGLUALA
12   PHEASPPHEVALLYSGLNARGARGGLYVAL
13   ILESERPROASNPHESERPHEMETGLYGLN
14   LEULEUGLNPHEGLUTHRGLNVALLEUCYS
15   HIS

Samples:

sample_1: MAP Kinase Phosphatase PAC-1, [U-99% 13C; U-99% 15N], 0.4 – 0.6 mM

Ex-cond_1: pH: 8.0; temperature: 298 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availableEx-cond_1
1H-15N TOCSYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HN(COCA)CBsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
C(CO)NHsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker . 500 MHz
  • Bruker . 600 MHz

Related Database Links:

BMRB 5552
PDB
GB AAA50779 AAA86112 AAH07771 AAY24222 ACE87048
REF NP_004409 XP_001111118 XP_002757414 XP_002811694 XP_003281084
SP Q05923
AlphaFold Q05923

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks