BMRB Entry 5116

Title:
Solution Structure of Pyrobaculum Aerophilum DsrC/gamma subunit of dissimilatory sulfite reductase (reduced)
Deposition date:
2001-08-17
Original release date:
2002-01-23
Authors:
Cort, J.; Kennedy, M.
Citation:

Citation: Cort, J.; Mariappan, S.V.; Kim, C.; Park, M.; Peat, T.; Waldo, G.; Terwilliger, T.; Kennedy, M.. "Solution Structure of Pyrobaculum aerophilum DsrC, an archaeal homologue of the gamma subunit of dissimilatory sulfite reductase"  Eur. J. Biochem. 268, 5842-5850 (2001).
PubMed: 11722571

Assembly members:

Assembly members:
gamma subunit of dissimilatory sulfite reductase, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 13773   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrobaculum aerophilum

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts91
13C chemical shifts173
15N chemical shifts91

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DsrC1

Entities:

Entity 1, DsrC 119 residues - Formula weight is not available

1   METPROVALLYSCYSPROGLYGLUTYRGLN
2   VALASPGLYLYSLYSVALILELEUASPGLU
3   ASPCYSPHEMETGLNASNPROGLUASPTRP
4   ASPGLULYSVALALAGLUTRPLEUALAARG
5   GLULEUGLUGLYILEGLNLYSMETTHRGLU
6   GLUHISTRPLYSLEUVALLYSTYRLEUARG
7   GLUTYRTRPGLUTHRPHEGLYTHRCYSPRO
8   PROILELYSMETVALTHRLYSGLUTHRGLY
9   PHESERLEUGLULYSILETYRGLNLEUPHE
10   PROSERGLYPROALAHISGLYALACYSLYS
11   VALALAGLYALAPROLYSPROTHRGLYCYS
12   VALGLYSERHISHISHISHISHISHIS

Samples:

13C-15N_DsrC_reduced: gamma subunit of dissimilatory sulfite reductase, [U-99% 13C; U-99% 15N], 1.5 – 2.0 mM; DTT 10 mM; sodium phosphate 25 mM; sodium chloride 100 mM

sample_cond_1: pH: 7.4 na; temperature: 298 K; ionic strength: 0.125 M; pressure: 1 atm

sample_cond_2: pH*: 7.0 na; temperature: 298 K; ionic strength: 0.125 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
4D 13C-separated NOESYnot availablenot availablenot available
3D 13C,15N-simultaneous NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
1H-15Nnot availablenot availablenot available
HSQC D2O exchangenot availablenot availablenot available
CBCACONNHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCC-TOCSY-NNHnot availablenot availablenot available
CCC-TOCSY-NNHnot availablenot availablenot available
CBCACOCAHAnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available

Software:

X-PLOR v3.84 - structure solution

FELIX v98 - processing

VNMR - collection

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 5115
PDB
GB AAL64285
REF NP_560103 WP_011008753

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks