BMRB Entry 5251

Title:
Complete backbone and partial side chain 1H, 15N and 13C assignments for human lymphotactin at 45 C
Deposition date:
2002-01-08
Original release date:
2002-06-13
Authors:
Kuloglu, E.; McCaslin, Darrell; Markley, John; Volkman, Brian
Citation:

Citation: Kuloglu, E.; McCaslin, Darrell; Markley, John; Volkman, Brian. "Structural Rearrangement of Human Lymphotactin, a C Chemokine, under Physiological Solution Conditions"  J. Biol. Chem. 277, 17863-17870 (2002).
PubMed: 11889129

Assembly members:

Assembly members:
lymphotactin, polymer, 93 residues, 10254 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts262
15N chemical shifts86
1H chemical shifts313

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lymphotactin, chain A1
2lymphotactin, chain B1

Entities:

Entity 1, lymphotactin, chain A 93 residues - 10254 Da.

1   VALGLYSERGLUVALSERASPLYSARGTHR
2   CYSVALSERLEUTHRTHRGLNARGLEUPRO
3   VALSERARGILELYSTHRTYRTHRILETHR
4   GLUGLYSERLEUARGALAVALILEPHEILE
5   THRLYSARGGLYLEULYSVALCYSALAASP
6   PROGLNALATHRTRPVALARGASPVALVAL
7   ARGSERMETASPARGLYSSERASNTHRARG
8   ASNASNMETILEGLNTHRLYSPROTHRGLY
9   THRGLNGLNSERTHRASNTHRALAVALTHR
10   LEUTHRGLY

Samples:

sample_1: lymphotactin, [U-13C; U-15N], 0.5 – 1.0 mM

sample_2: lymphotactin, [U-15N], 0.5 – 1.0 mM

Ex-cond_1: pH: 6.0; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
15N NOESYnot availablenot availablenot available
15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
13C NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

BMRB 15215 5042
PDB
DBJ BAA07825 BAA09859
EMBL CAA60198 CAB46691
GenBank AAC50164 AAH69817 AAH70309 EAW90827 EAW90828
REF NP_002986 XP_524955
SWISS-PROT P47992

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks