BMRB Entry 5660

Title:
Partial proton resonance assignements for the different conformers of ferricytochrome c produced in 30% acetonitrile and 70% water solution
Deposition date:
2003-01-14
Original release date:
2003-02-25
Authors:
Sivakolundu, Sivashankar; Mabrouk, Patricia
Citation:

Citation: Sivakolundu, Sivashankar; Mabrouk, Patricia. "Insights into the Alkaline Transformation of Ferricyt c from 1H NMR Study in 30% Acetonitrile-Water"  Protein Sci. 10, 2291-2300 (2001).

Assembly members:

Assembly members:
oxidized cytochrome c, polymer, 104 residues, 12384 Da.
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: Horse   Taxonomy ID: 9796   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:

Experimental source:   Production method: vendor

Entity Sequences (FASTA):

Data typeCount
1H chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c1
2HEME C2

Entities:

Entity 1, cytochrome c 104 residues - 12384 Da.

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   VALGLNLYSCYSALAGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYPHETHRTYRTHRASP
6   ALAASNLYSASNLYSGLYILETHRTRPLYS
7   GLUGLUTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEALAGLYILELYSLYSLYSTHRGLU
10   ARGGLUASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, HEME C - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: oxidized cytochrome c 2 mM; PROTOPORPHYRIN IX CONTAINING FE 2 mM; acetonitrile 30%; H2O 70%

Ex-cond_1: pH*: 7.2; temperature: 293 K

Ex-cond_2: pH*: 7.2; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H NOESYsample_1not availablenot available

Software:

FELIX v2000 - spectral processing, spectral analysis

NMR spectrometers:

  • Varian Mercury 300 MHz
  • home made . 500 MHz
  • home made . 600 MHz

Related Database Links:

SWISS-PROT P68097 P68096 P00004
REF XP_001498872
PRF 610169A
GenBank AAB33495
PDB
BMRB 948 947 946 944 673 672 665 645 630 5830 5829 5828 5827 546 545 544 543 5372 5026 499 4810 4809 4808 4805 439 438 437 436 4189 336 317 316 286 285 274 244 243 2368 2367 2366 224 220 216 1789 1787 1785 1783 1736 1404 1171 1170 1116 1114 1113 1112 1111 1110 1109 1108 1107 1058