BMRB Entry 5782

Title:
1H chemical shift assignment for the truncated form of the human beta2-microglobulin lacking of the first 3 residues
Deposition date:
2003-04-24
Original release date:
2004-02-12
Authors:
Viglino, Paolo; Corazza, Alessandra; Pettirossi, Fabio; Verdone, Giuliana; Esposito, Gennaro
Citation:

Citation: Corazza, Alessandra; Pettirossi, Fabio; Viglino, Paolo; Verdone, Giuliana; Garcia, Julian; Dumy, Pascal; Giorgetti, Sofia; Mangione, Palma; Andreola, Alessia; Stoppini, Monica; Bellotti, Vittorio; Esposito, Gennaro. "Properties of some variants of human beta2-microglobulin and amyloidogenesis"  J. Biol. Chem. ., .-..
PubMed: 14660575

Assembly members:

Assembly members:
beta2-microglobulin, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts716

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DN3beta2-microglobulin1

Entities:

Entity 1, DN3beta2-microglobulin 97 residues - Formula weight is not available

1   METTHRPROLYSILEGLNVALTYRSERARG
2   HISPROALAGLUASNGLYLYSSERASNPHE
3   LEUASNCYSTYRVALSERGLYPHEHISPRO
4   SERASPILEGLUVALASPLEULEULYSASN
5   GLYGLUARGILEGLULYSVALGLUHISSER
6   ASPLEUSERPHESERLYSASPTRPSERPHE
7   TYRLEULEUTYRTYRTHRGLUPHETHRPRO
8   THRGLULYSASPGLUTYRALACYSARGVAL
9   ASNHISVALTHRLEUSERGLNPROLYSILE
10   VALLYSTRPASPARGASPMET

Samples:

sample_1: beta2-microglobulin 0.86 mM; sodium phosphate 70 mM; sodium chloride 100 mM; H2O 100%

sample_2: beta2-microglobulin 0.7 mM; sodium phosphate 70 mM; sodium chloride 100 mM; D2O 95%; H2O 5%

Ex-cond_1: pH: 6.5 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-1H TOCSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
1H-1H DQF-COSYnot availablenot availablenot available

Software:

FELIX v2000 -

NMR spectrometers:

  • Bruker Avance 500 MHz