BMRB Entry 5951

Title:
1H and 15N chemical shift assignment and secondary structure of human saposin C
Deposition date:
2003-09-16
Original release date:
2005-10-24
Authors:
John, Michael; Patard, Louis; Wendeler, Michaela; Sandhoff, Konrad; Kessler, Horst
Citation:

Citation: John, Michael; Patard, Louis; Wendeler, Michaela; Sandhoff, Konrad; Kessler, Horst. "1H and 15N chemical shift assignment and secondary structure of human saposin C"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
saposin C, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts75
1H chemical shifts521

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SAP-C1

Entities:

Entity 1, SAP-C 87 residues - Formula weight is not available

1   SERASPVALTYRCYSGLUVALCYSGLUPHE
2   LEUVALLYSGLUVALTHRLYSLEUILEASP
3   ASNASNLYSTHRGLULYSGLUILELEUASP
4   ALAPHEASPLYSMETCYSSERLYSLEUPRO
5   LYSSERLEUSERGLUGLUCYSGLNGLUVAL
6   VALASPTHRTYRGLYSERSERILELEUSER
7   ILELEULEUGLUGLUVALSERPROGLULEU
8   VALCYSSERMETLEUHISLEUCYSSERGLY
9   ARGHISHISHISHISHISHIS

Samples:

sample_1: saposin C, [U-15N], 1.4 mM; sodium phosphate 50 mM

sample_2: saposin C, [U-15N], 1.4 mM; sodium acetate 50 mM

pH7: pH: 7.0; temperature: 310 K

pH4: pH: 4.0; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
15N HSQC-NOESY-HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 5465 6158
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks