BMRB Entry 6026

Title:
Chemical Shift Assignments for Oxidized Human Ferredoxin
Deposition date:
2003-12-01
Original release date:
2004-07-30
Authors:
Machonkin, Timothy; Westler, William; Markley, John
Citation:

Citation: Machonkin, Timothy; Westler, William; Markley, John. "Strategy for the Study of Paramagnetic Proteins with Slow Electronic Relaxation Rates by NMR Spectroscopy: Application to Oxidized Human [2Fe-2S] Ferredoxin"  J. Am. Chem. Soc. 126, 5413-5426 (2004).
PubMed: 15113213

Assembly members:

Assembly members:
Oxidized Human Ferredoxin, polymer, 124 residues, Formula weight is not available
FES, non-polymer, 175.820 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts585
13C chemical shifts401
15N chemical shifts16

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ferredoxin1
2[2Fe-2S] cluster2

Entities:

Entity 1, Ferredoxin 124 residues - Formula weight is not available

1   SERSERSERGLUASPLYSILETHRVALHIS
2   PHEILEASNARGASPGLYGLUTHRLEUTHR
3   THRLYSGLYLYSVALGLYASPSERLEULEU
4   ASPVALVALVALGLUASNASNLEUASPILE
5   ASPGLYPHEGLYALACYSGLUGLYTHRLEU
6   ALACYSSERTHRCYSHISLEUILEPHEGLU
7   ASPHISILETYRGLULYSLEUASPALAILE
8   THRASPGLUGLUASNASPMETLEUASPLEU
9   ALATYRGLYLEUTHRASPARGSERARGLEU
10   GLYCYSGLNILECYSLEUTHRLYSSERMET
11   ASPASNMETTHRVALARGVALPROGLUTHR
12   VALALAASPALAARGGLNSERILEASPVAL
13   GLYLYSTHRSER

Entity 2, [2Fe-2S] cluster - Fe2 S2 - 175.820 Da.

1   FES

Samples:

sample_1: Oxidized Human Ferredoxin, [U-13C; U-15N], 4.4 mM; oxidized high-spin 2Fe-2S iron-sulfur cluster 4.4 mM; Phosphate buffer 50 mM; D2O 10%; H2O 90%; DSS 250 uM

sample_2: Oxidized Human Ferredoxin, [13C; 15N]-Cys, 6.1 mM; oxidized high-spin 2Fe-2S iron-sulfur cluster 6.1 mM; Phosphate buffer 50 mM; D2O 10%; H2O 90%; DSS 250 uM

sample_3: Oxidized Human Ferredoxin, [13Cb]-Cys, 1.0 mM; oxidized high-spin 2Fe-2S iron-sulfur cluster 1.0 mM; Phosphate buffer 50 mM; D2O 10%; H2O 90%; DSS 250 uM

sample_4: Oxidized Human Ferredoxin, [U-15N], 2.2 mM; oxidized high-spin 2Fe-2S iron-sulfur cluster 2.2 mM; Phosphate buffer 50 mM; D2O 10%; H2O 90%; DSS 250 uM

sample_5: Oxidized Human Ferredoxin, [15N]-Ala, 5.5 mM; oxidized high-spin 2Fe-2S iron-sulfur cluster 5.5 mM; Phosphate buffer 50 mM; D2O 10%; H2O 90%; DSS 250 uM

sample_6: Oxidized Human Ferredoxin, [15N]-Leu, 5.4 mM; oxidized high-spin 2Fe-2S iron-sulfur cluster 5.4 mM; Phosphate buffer 50 mM; D2O 10%; H2O 90%; DSS 250 uM

sample_7: Oxidized Human Ferredoxin, [15N]-Gly, 4.4 mM; oxidized high-spin 2Fe-2S iron-sulfur cluster 4.4 mM; Phosphate buffer 50 mM; D2O 10%; H2O 90%; DSS 250 uM

sample_8: Oxidized Human Ferredoxin, [15N]-Thr, 4.7 mM; oxidized high-spin 2Fe-2S iron-sulfur cluster 4.7 mM; Phosphate buffer 50 mM; D2O 10%; H2O 90%; DSS 250 uM

Ex-cond_1: pH: 7.35; temperature: 293 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
1D 1H-SuperWEFTnot availablenot availableEx-cond_1
1D 13C-SuperWEFTnot availablenot availableEx-cond_1
1D 15N 1-pulsenot availablenot availableEx-cond_1
1D 15N-SuperWEFTnot availablenot availableEx-cond_1
1D 1H{13C} Difference Decouplingnot availablenot availableEx-cond_1
1D 13C{15N} Difference Decouplingnot availablenot availableEx-cond_1
2D 1H{13C} HSQCnot availablenot availableEx-cond_1
3D 1H{13C} HC(C)H-COSYnot availablenot availableEx-cond_1
2D 13C{13C} CT-COSYnot availablenot availableEx-cond_1
2D 1H{13C} PRE-HSQCnot availablenot availableEx-cond_1
3D 1H{13C} (H)CCH-COSYnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

REF XP_508877 NP_004100 XP_001105034
SWISS-PROT P10109
GenBank AAA50462 AAA76853 AAA35855 AAA35856 AAA35829
BMRB 5337 4439 4440 4073 4074