BMRB Entry 6257

Title:
Alzheimer's Abeta40 Studied by NMR at Low pH Reveals That DSS Binds and Promotes beta-ball Oligomerization
Deposition date:
2004-06-30
Original release date:
2005-02-10
Authors:
Laurents, Douglas; Gorman, Paul; Guo, Ming; Rico, Manuel; Chakrabartty, Avijit; Bruix, Marta
Citation:

Citation: Laurents, Douglas; Gorman, Paul; Guo, Ming; Rico, Manuel; Chakrabartty, Avijit; Bruix, Marta. "Alzheimer's Abeta40 Studied by NMR at Low pH Reveals That DSS Binds and Promotes beta-ball Oligomerization"  J. Biol. Chem. 280, 3675-3685 (2004).
PubMed: 15557279

Assembly members:

Assembly members:
Abeta(1-40), polymer, 40 residues, 4300 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Abeta(1-40): DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV

Data sets:
Data typeCount
13C chemical shifts97
1H chemical shifts279

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Abeta(1-40)1

Entities:

Entity 1, Abeta(1-40) 40 residues - 4300 Da.

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAGLUASPVALGLYSERASNLYSGLYALA
4   ILEILEGLYLEUMETVALGLYGLYVALVAL

Samples:

sample_1: Abeta(1-40)0.15 – 0.30 mM; NaH2PO4 5 mM

Ex-cond_1: ionic strength: 0.01 M; pH: 2.5; temperature: 298 K

Ex-cond_2: ionic strength: 0.01 M; pH*: 2.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
COSYnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available

Software:

UXWINNMR -

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 5400 6554
PDB
DBJ BAA22264 BAA84580 BAD51938 BAE01907 BAG10647
EMBL CAA30050 CAA31830 CAA39589 CAA39590 CAA39591
GenBank AAA35540 AAA36829 AAA51564 AAA51722 AAA51726
PIR A60045 D60045 E60045 G60045 PQ0438
PRF 1303338A 1403400A 1405204A 1507304A 1507304B
REF NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_958816
SWISS-PROT P05067 P53601 P79307 Q28053 Q28280