BMRB Entry 6258

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6258

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N and Cb chemical shift assignments for TolAIII in complex with g3pN1

Deposition date:

2004-06-30

Original release date:

2005-02-21

Authors:

Deprez, Christophe; Lloubes, Roland; Gavioli, Marthe; Marion, Dominique; Guerlesquin, Francoise; Blanchard, Laurence

Citation:

Citation: Deprez, Christophe; Lloubes, Roland; Gavioli, Marthe; Marion, Dominique; Guerlesquin, Francoise; Blanchard, Laurence. "Solution Structure of the E.coli TolA C-terminal Domain Reveals Conformational Changes upon Binding to the Phage g3p N-terminal Domain" J. Mol. Biol. 346, 1047-1057 (2005).
PubMed: 15701516

Assembly members:

Assembly members:
TolA domain III, polymer, 106 residues, Formula weight is not available
g3pN1, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia E. coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TolA domain III: AEFGNTKNNGASGADINNYA GQIKSAIESKFYDASSYAGK TCTLRIKLAPDGMLLDIKPE GGDPALCQAALAAAKLAKIP KPPSQAVYEVFKNAPLDFKP HHHHHH
g3pN1: MKKLLFAIPLVVPFYSHSAE TVESCLAKPHTENSFTNVWK DDKTLDRYANYEGCLWNATG VVVCTGDETQCHHHHHH

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	225
15N chemical shifts	80
1H chemical shifts	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TolAIII	1
2	g3pN1	2

Entities:

Entity 1, TolAIII 106 residues - Formula weight is not available

1

ALA

GLU

PHE

GLY

ASN

THR

LYS

ASN

GLY

2

ALA

SER

GLY

ALA

ASP

ILE

ASN

TYR

ALA

3

GLY

GLN

ILE

LYS

SER

ALA

ILE

GLU

SER

LYS

4

PHE

TYR

ASP

ALA

SER

TYR

ALA

GLY

LYS

5

THR

CYS

THR

LEU

ARG

ILE

LYS

LEU

ALA

PRO

6

ASP

GLY

MET

LEU

ASP

ILE

LYS

PRO

GLU

7

GLY

ASP

PRO

ALA

LEU

CYS

GLN

ALA

8

LEU

ALA

LYS

LEU

ALA

LYS

ILE

PRO

9

LYS

PRO

SER

GLN

ALA

VAL

TYR

GLU

VAL

10

PHE

LYS

ASN

ALA

PRO

LEU

ASP

PHE

LYS

PRO

11

HIS

Entity 2, g3pN1 77 residues - Formula weight is not available

1

MET

LYS

LEU

PHE

ALA

ILE

PRO

LEU

2

VAL

PRO

PHE

TYR

SER

HIS

SER

ALA

GLU

3

THR

VAL

GLU

SER

CYS

LEU

ALA

LYS

PRO

HIS

4

THR

GLU

ASN

SER

PHE

THR

ASN

VAL

TRP

LYS

5

ASP

LYS

THR

LEU

ASP

ARG

TYR

ALA

ASN

6

TYR

GLU

GLY

CYS

LEU

TRP

ASN

ALA

THR

GLY

7

VAL

CYS

THR

GLY

ASP

GLU

THR

GLN

8

CYS

HIS

Samples:

sample_1: TolA domain III, [U-13C; U-15N], 0.4 mM; g3pN1 0.6 mM; potassium phosphate buffer 30 mM; NaCl 30 mM

cond_1: ionic strength: 60 mM; pH: 6.8; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	not available
3D HNCA	not available	not available	not available
3D DEPT-CBCA(CO)NH	not available	not available	not available
3D HNCO	not available	not available	not available

Software:

FELIX -

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz

BMRB	4771
PDB	1S62 1TOL 1FGP 2G3P
DBJ	BAA35405 BAB34197 BAF74208 BAF74218
GenBank	AAA24683 AAC73833 AAG55075 AAN42202 AAN79291 AAA32215 AAA32309 AAF75609 AAM73889 AAM73901
REF	AP_001377 NP_286467 NP_308801 NP_415267 NP_706495 NP_510891
SWISS-PROT	P19934 P03661 P69168 P69169
EMBL	CAA23862 CAA23872
PIR	Z3BPF1
PRF	0812197D