BMRB Entry 7135

Title:
Assignments of 2SSalpha in 8M urea, pH 2, 293 K
Deposition date:
2006-06-01
Original release date:
2008-11-12
Authors:
Collins, Emily; Wirmer, Julia; Hirai, Kenichi; Tachibana, Hideki; Segawa, Shin-ichi; Dobson, Christopher; Schwalbe, Harald
Citation:

Citation: Collins, Emily; Wirmer, Julia; Hirai, Kenichi; Tachibana, Hideki; Segawa, Shin-ichi; Dobson, Christopher; Schwalbe, Harald. "Characterisation of Disulfide Bond Dynamics in Non-Native States of Lysozyme and its Disulfide Deletion Mutants by NMR"  ChemBioChem 6, 1619-1627 (2005).
PubMed: 16138305

Assembly members:

Assembly members:
2SS(alpha), polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Hen   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
15N chemical shifts108
1H chemical shifts216

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
12SS(alpha)1

Entities:

Entity 1, 2SS(alpha) 129 residues - Formula weight is not available

1   LYSVALPHEGLYARGCYSGLULEUALAALA
2   ALAMETLYSARGHISGLYLEUASPASNTYR
3   ARGGLYTYRSERLEUGLYASNTRPVALCYS
4   ALAALALYSPHEGLUSERASNPHEASNTHR
5   GLUALATHRASNARGASNTHRASPGLYSER
6   THRASPTYRGLYILELEUGLNILEASNSER
7   ARGTRPTRPALAASNASPGLYARGTHRPRO
8   GLYSERARGASNLEUALAASNILEPROALA
9   SERALALEULEUSERSERASPILETHRALA
10   SERVALASNALAALALYSLYSILEVALSER
11   ASPGLYASNGLYMETASNALATRPVALALA
12   TRPARGASNARGCYSLYSGLYTHRASPVAL
13   GLUALATRPILEARGGLYCYSARGLEU

Samples:

sample_1: 2SS(alpha) 1 mM; urea 8 M; D2O 10%

conditions_1: pH: 2; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
unknownsample_1isotropicconditions_1

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 11459 11460 5069 5803 5804 7159
PDB

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