BMRB Entry 7250

Title:
Backbone 1H, 13C, 15N Chemical Shift Assignments for HIV-1 unmyristoylated matrix protein
Deposition date:
2006-08-04
Original release date:
2006-08-15
Authors:
Saad, Jamil; Miller, Jaime; Tai, Janet; Kim, Andrew; Ghanam, Ruba; Summers, Michael
Citation:

Citation: Saad, Jamil; Miller, Jaime; Tai, Janet; Kim, Andrew; Ghanam, Ruba; Summers, Michael. "Structural Basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assembly"  Proc. Natl. Acad. Sci. U.S.A. 103, 11364-11369 (2006).
PubMed: 16840558

Assembly members:

Assembly members:
Matrix protein, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts429
15N chemical shifts131
1H chemical shifts782

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HIV-1 unmyristoylated Matrix1

Entities:

Entity 1, HIV-1 unmyristoylated Matrix 131 residues - Formula weight is not available

1   GLYALAARGALASERVALLEUSERGLYGLY
2   GLULEUASPLYSTRPGLULYSILEARGLEU
3   ARGPROGLYGLYLYSLYSGLNTYRLYSLEU
4   LYSHISILEVALTRPALASERARGGLULEU
5   GLUARGPHEALAVALASNPROGLYLEULEU
6   GLUTHRSERGLUGLYCYSARGGLNILELEU
7   GLYGLNLEUGLNPROSERLEUGLNTHRGLY
8   SERGLUGLULEUARGSERLEUTYRASNTHR
9   ILEALAVALLEUTYRCYSVALHISGLNARG
10   ILEASPVALLYSASPTHRLYSGLUALALEU
11   ASPLYSILEGLUGLUGLUGLNASNLYSSER
12   LYSLYSLYSALAGLNGLNALAALAALAASP
13   THRGLYASNASNSERGLNVALSERGLNASN
14   TYR

Samples:

sample_1: Matrix protein, [U-15 N; U-13 C], 0.5 mM

conditions_1: pH: 5.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1not availableconditions_1
3D 15N-edited NOESYsample_1not availableconditions_1
13C-edited NOESYsample_1not availableconditions_1
4D 15N,13C-edited NOESYsample_1not availableconditions_1
4D 13C,13C-edited NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15114 15116 18715 18716 5316 5960 7267 7275 7309
PDB
DBJ BAF34641 BAG48474
EMBL CBI61180 CBI61181 CBI61182 CBI61183 CBI61184
GB AAA44987 AAB00898 AAB60571 AAC28445 AAC29216
SP P12493 P12497
AlphaFold P12493 P12497

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks