BMRB Entry 10019

Title:
GSPT1/eRF3a(PAM2-1)-PABC complex
Deposition date:
2006-03-27
Original release date:
2010-05-27
Authors:
Osawa, Masanori; Shimada, Ichio
Citation:

Citation: Osawa, Masanori; Shimada, Ichio. "1H and 15N Assigned Chemical Shifts for GSPT1/eRF3a(PAM2-1) and 1H, 13C and 15N Assigned Chemical Shifts for PABC"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
PAM2-1, polymer, 19 residues, Formula weight is not available
PABC domain, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculas

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts257
15N chemical shifts77
1H chemical shifts674

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAM2-11
2PABC2

Entities:

Entity 1, PAM2-1 19 residues - Formula weight is not available

1   ALAPHESERARGGLNLEUASNVALASNALA
2   LYSPROPHEVALPROASNVALHISALA

Entity 2, PABC 83 residues - Formula weight is not available

1   GLYGLNGLUPROLEUTHRALASERMETLEU
2   ALASERALAPROPROGLNGLUGLNLYSGLN
3   METLEUGLYGLUARGLEUPHEPROLEUILE
4   GLNALAMETHISPROTHRLEUALAGLYLYS
5   ILETHRGLYMETLEULEUGLUILEASPASN
6   SERGLULEULEUHISMETLEUGLUSERPRO
7   GLUSERLEUARGSERLYSVALASPGLUALA
8   VALALAVALLEUGLNALAHISGLNALALYS
9   GLUALAALA

Samples:

sample_1: PAM2-1, unlabeled, 1 mM; PABC domain, [U-95% 13C, U-95% 15N], 1 mM; NaCl 150 mM

conditions_1: ionic strength: 0.15 M; pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N-1H HSQCsample_1not availableconditions_1
13C-1H HSQCsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
C(CO)NHsample_1not availableconditions_1
H(CCO)NHsample_1not availableconditions_1
HBHA(CBCACO)NHsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
15N-edited TOCSY HSQCsample_1not availableconditions_1
15N-edited NOESY HSQCsample_1not availableconditions_1
13C,15N-filterd NOESYsample_1not availableconditions_1
13C,15N-filterd TOCSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAA92160 BAE22025 BAJ20641 BAT46595
EMBL CAG31442 CAH93403
GB AAH09503 AAH31640 AAH77825 AAH79092 AAH90081
REF NP_001003978 NP_001015805 NP_001086960 NP_001091540 NP_001123478
SP Q5R4B3 Q8R050
TPG DAA15601
AlphaFold Q5R4B3 Q8R050

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks