BMRB Entry 10054

Title:
Solution structure of the first cold-shock domain of the human KIAA0885 protein (UNR protein)
Deposition date:
2006-11-29
Original release date:
2008-08-13
Authors:
Goroncy, A.; Kigawa, T.; Koshiba, S.; Tomizawa, T.; Kobayashi, N.; Tochio, N.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Goroncy, A.; Kigawa, T.; Koshiba, S.; Tomizawa, T.; Kobayashi, N.; Tochio, N.; Inoue, M.; Yokoyama, S.. "Solution structure of the first cold-shock domain of the human KIAA0885 protein (UNR protein)"  .

Assembly members:

Assembly members:
First cold-shock domain, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040114-45

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts96
1H chemical shifts589

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UNR protein1

Entities:

Entity 1, UNR protein 89 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYTYRPRO
2   ASNGLYTHRSERALAALALEUARGGLUTHR
3   GLYVALILEGLULYSLEULEUTHRSERTYR
4   GLYPHEILEGLNCYSSERGLUARGGLNALA
5   ARGLEUPHEPHEHISCYSSERGLNTYRASN
6   GLYASNLEUGLNASPLEULYSVALGLYASP
7   ASPVALGLUPHEGLUVALSERSERASPARG
8   ARGTHRGLYLYSPROILEALAVALLYSLEU
9   VALLYSILESERGLYPROSERSERGLY

Samples:

sample_1: cold-shock domain, [U-13C; U-15N], 1.09 mM; phosphate 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, BRUKER - collection

NMRPipe v20031121, DELAGLIO, FRANK - processing

NMRView v5.0.14, JOHNSON, BRUCE, A. - data analysis

Kujira v0.899, KOBAYASHI, NAOHIRO - data analysis

CYANA v2.0.17, GUENTERT, P. - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAE01840

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks