BMRB Entry 10063

Title:
Solution structure of the fourth SH3 domain of human intersectin 2 (KIAA1256)
Deposition date:
2006-12-25
Original release date:
2008-08-14
Authors:
Tochio, N.; Kigawa, T.; Koshiba, S.; Kobayashi, N.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Kigawa, T.; Koshiba, S.; Kobayashi, N.; Inoue, M.; Yokoyama, S.. "Solution structure of the fourth SH3 domain of human intersectin 2 (KIAA1256)"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P021015-65

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts304
15N chemical shifts74
1H chemical shifts482

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Intersectin 21

Entities:

Entity 1, Intersectin 2 80 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUILEALA
2   GLNVALTHRSERALATYRVALALASERGLY
3   SERGLUGLNLEUSERLEUALAPROGLYGLN
4   LEUILELEUILELEULYSLYSASNTHRSER
5   GLYTRPTRPGLNGLYGLULEUGLNALAARG
6   GLYLYSLYSARGGLNLYSGLYTRPPHEPRO
7   ALASERHISVALLYSLEULEUGLYPROSER
8   SERGLUARGALASERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.4 mM; phosphate 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks