BMRB Entry 10251

Title:
Solution structure of the C-terminal PH domain of human pleckstrin 2
Deposition date:
2008-11-20
Original release date:
2009-11-20
Authors:
Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C-terminal PH domain of human pleckstrin 2"  .

Assembly members:

Assembly members:
PH domain, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: Cell free synthesis   Vector: P040614-11

Data sets:
Data typeCount
13C chemical shifts550
15N chemical shifts124
1H chemical shifts855

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PH domain1

Entities:

Entity 1, PH domain 129 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERLEUSER
2   THRVALGLULEUSERGLYTHRVALVALLYS
3   GLNGLYTYRLEUALALYSGLNGLYHISLYS
4   ARGLYSASNTRPLYSVALARGARGPHEVAL
5   LEUARGLYSASPPROALAPHELEUHISTYR
6   TYRASPPROSERLYSGLUGLUASNARGPRO
7   VALGLYGLYPHESERLEUARGGLYSERLEU
8   VALSERALALEUGLUASPASNGLYVALPRO
9   THRGLYVALLYSGLYASNVALGLNGLYASN
10   LEUPHELYSVALILETHRLYSASPASPTHR
11   HISTYRTYRILEGLNALASERSERLYSALA
12   GLUARGALAGLUTRPILEGLUALAILELYS
13   LYSLEUTHRSERGLYPROSERSERGLY

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.24 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.913, Kobayashi, N. - data analysis

CYANA v1.0.7, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
REF XP_011384462 XP_011534947 XP_011724706

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks