BMRB Entry 10282

Title:
Solution structure of the homeobox domain of the human hypothetical protein FLJ21616
Deposition date:
2008-12-17
Original release date:
2009-12-17
Authors:
Ohnishi, S.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Ohnishi, S.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the homeobox domain of the human hypothetical protein FLJ21616"  .

Assembly members:

Assembly members:
homeobox domain, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P041004-01

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts380
15N chemical shifts89
1H chemical shifts581

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 95 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGGLYSER
2   ARGPHETHRTRPARGLYSGLUCYSLEUALA
3   VALMETGLUSERTYRPHEASNGLUASNGLN
4   TYRPROASPGLUALALYSARGGLUGLUILE
5   ALAASNALACYSASNALAVALILEGLNLYS
6   PROGLYLYSLYSLEUSERASPLEUGLUARG
7   VALTHRSERLEULYSVALTYRASNTRPPHE
8   ALAASNARGARGLYSGLUILELYSARGARG
9   ALAASNILEALAALAILELEUGLUSERSER
10   GLYPROSERSERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.40 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert. P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB15099 BAC35118 BAC40961 BAF83372 BAG58297
GB AAH02212 AAH09259 AAH51457 AAH69242 AAH77394
REF NP_001086952 NP_001087099 NP_001129198 NP_001179100 NP_001264645
SP Q6NT76 Q8BJA3
TPG DAA27058
AlphaFold Q6NT76 Q8BJA3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks