BMRB Entry 10296

Title:
Solution structure of the homeobox domain of Homeobox protein goosecoid
Deposition date:
2008-12-17
Original release date:
2009-12-17
Authors:
Ohnishi, S.; Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Ohnishi, S.; Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the homeobox domain of Homeobox protein goosecoid"  .

Assembly members:

Assembly members:
homeobox domain, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P050919-23

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts280
15N chemical shifts63
1H chemical shifts423

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 70 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGARGHIS
2   ARGTHRILEPHETHRASPGLUGLNLEUGLU
3   ALALEUGLUASNLEUPHEGLNGLUTHRLYS
4   TYRPROASPVALGLYTHRARGGLUGLNLEU
5   ALAARGLYSVALHISLEUARGGLUGLULYS
6   VALGLUVALTRPPHELYSASNARGARGALA
7   LYSTRPARGARGSERGLYPROSERSERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.3 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.965, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
DBJ BAG73208 BAK40073 BAR79454
EMBL CAA49897 CAA73611 CAA73612 CAA76833 CAJ82436
GB AAA37826 AAA49729 AAA49744 AAA50028 AAA50030
REF NP_001016704 NP_001074955 NP_001081278 NP_001093429 NP_001107361
SP A1YEY5 A1YFI3 A1YG57 A2T733 A2T7P4
TPG DAA17416
AlphaFold A1YEY5 A1YFI3 A1YG57 A2T733 A2T7P4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks