BMRB Entry 11073

Title:
Solution structure of juvenile hormone binding protein from silkworm in complex with JH III
Deposition date:
2009-04-27
Original release date:
2009-10-14
Authors:
Suzuki, Rintaro; Fujimoto, Zui; Shiotsuki, Takahiro; Momma, Mitsuru; Tase, Akira; Yamazaki, Toshimasa
Citation:

Citation: Suzuki, Rintaro; Fujimoto, Zui; Shiotsuki, Takahiro; Momma, Mitsuru; Tase, Akira; Miyazawa, Mitsuhiro; Yamazaki, Toshimasa. "Structural basis for juvenile hormone perception by JHBP in haemolymph"  .

Assembly members:

Assembly members:
hJHBP, polymer, 227 residues, 24781.268 Da.
JH3, non-polymer, 266.376 Da.

Natural source:

Natural source:   Common Name: Silkworm   Taxonomy ID: 7091   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bombyx mori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Data sets:
Data typeCount
13C chemical shifts1049
15N chemical shifts242
1H chemical shifts1635

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hJHBP1
2JH III2

Entities:

Entity 1, hJHBP 227 residues - 24781.268 Da.

Residues (-2)-(-1) represent a non-native sequence from affinity tag.

1   GLYSERASPGLYASPALALEULEULYSPRO
2   CYSLYSLEUGLYASPMETGLNCYSLEUSER
3   SERALATHRGLUGLNPHELEUGLULYSTHR
4   SERLYSGLYILEPROGLNTYRASPILETRP
5   PROILEASPPROLEUVALVALTHRSERLEU
6   ASPVALILEALAPROSERASPALAGLYILE
7   VALILEARGPHELYSASNLEUASNILETHR
8   GLYLEULYSASNGLNGLNILESERASPPHE
9   GLNMETASPTHRLYSALALYSTHRVALLEU
10   LEULYSTHRLYSALAASPLEUHISILEVAL
11   GLYASPILEVALILEGLULEUTHRGLUGLN
12   SERLYSSERPHETHRGLYLEUTYRTHRALA
13   ASPTHRASNVALILEGLYALAVALARGTYR
14   GLYTYRASNLEULYSASNASPASPASNGLY
15   VALGLNHISPHEGLUVALGLNPROGLUTHR
16   PHETHRCYSGLUSERILEGLYGLUPROLYS
17   ILETHRLEUSERSERASPLEUSERSERALA
18   LEUGLULYSASPSERGLYASNASNSERLEU
19   GLUPROASPMETGLUPROLEULYSTHRLEU
20   ARGGLNALAALAILECYSLYSILEALAGLU
21   ALACYSTYRILESERVALVALHISASNILE
22   ARGALASERALALYSILELEUPROALASER
23   SERPHEPHEGLUASNLEUASN

Entity 2, JH III - C16 H26 O3 - 266.376 Da.

1   JH3

Samples:

15N: hJHBP, [U-15N], 0.6 – 1.0 mM; JH III0.6 – 1.0 mM; sodium phosphate 45 mM; H2O 90%; D2O 10%

13C_15N: hJHBP, [U-13C; U-15N], 0.6 – 1.0 mM; JH III0.6 – 1.0 mM; sodium phosphate 45 mM; H2O 90%; D2O 10%

13C_15N_in_100%_D2O: hJHBP, [U-13C; U-15N], 0.6 – 1.0 mM; JH III0.6 – 1.0 mM; sodium phosphate 45 mM; D2O 100%

pH_6.0_35C: pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15NisotropicpH_6.0_35C
2D 1H-15N HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C
2D 1H-13C HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D HNCO13C_15NisotropicpH_6.0_35C
3D CBCA(CO)NH13C_15NisotropicpH_6.0_35C
3D HNCA13C_15NisotropicpH_6.0_35C
3D HCABGCO13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D HCCH-TOCSY13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D 15N-separated HOHAHA-HSQC15NisotropicpH_6.0_35C
3D 15N-separated NOESY-HSQC15NisotropicpH_6.0_35C
3D 13C/15N-separated NOESY-HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D 13C-separated NOESY-HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D 13C/13C-separated NOESY-HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C
2D 13C-filtered COSY13C_15N_in_100%_D2OisotropicpH_6.0_35C
2D 13C-filtered NOESY13C_15N_in_100%_D2OisotropicpH_6.0_35C
3D 13C-filtered/13C-selected NOESY-HSQC13C_15N_in_100%_D2OisotropicpH_6.0_35C

Software:

xwinnmr v3.1, Bruker Biospin - collection

NMRPipe vreleased at Feb 10, 2006, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

TALOS v2003.027.13.05, Cornilescu, Delaglio and Bax - data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

AQUA v3.2, Rullmann, Doreleijers and Kaptein - data analysis

ProcheckNMR v3.5.4, Laskowski and MacArthur - data analysis

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

GB AF098304 AAF19267 AAF19268 AAP41112
SWS Q9U556_BOMMO
BMRB 16021
PDB
REF NP_001037074

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks