BMRB Entry 11097

Title:
Solution structure of the fifth fibronectin type III domain of human Netrin receptor DCC
Deposition date:
2010-02-19
Original release date:
2011-02-17
Authors:
Tochio, N.; Koshiba, S.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Koshiba, S.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fifth fibronectin type III domain of human Netrin receptor DCC"  To be Published ., .-..

Assembly members:

Assembly members:
fifth fn3 domain, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050905-23

Data sets:
Data typeCount
13C chemical shifts500
15N chemical shifts111
1H chemical shifts747

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fifth fn3 domain1

Entities:

Entity 1, fifth fn3 domain 123 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPHEPROTHR
2   SERVALPROASPLEUSERTHRPROMETLEU
3   PROPROVALGLYVALGLNALAVALALALEU
4   THRHISASPALAVALARGVALSERTRPALA
5   ASPASNSERVALPROLYSASNGLNLYSTHR
6   SERGLUVALARGLEUTYRTHRVALARGTRP
7   ARGTHRSERPHESERALASERALALYSTYR
8   LYSSERGLUASPTHRTHRSERLEUSERTYR
9   THRALATHRGLYLEULYSPROASNTHRMET
10   TYRGLUPHESERVALMETVALTHRLYSASN
11   ARGARGSERSERTHRTRPSERMETTHRALA
12   HISALATHRTHRTYRGLUALASERGLYPRO
13   SERSERGLY

Samples:

sample_1: fifth fn3 domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl, [U-2H], 20 mM; sodium chloride 100 mM; d-DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropiccondition_1
3D 1H-13C NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMNMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.955, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAG63884 BAI45499
EMBL CAA53735
GB AAI52809 EAW62991 EAW62994 EFB26812 EHB03455
REF NP_005206 XP_001916613 XP_002757292 XP_002800982 XP_002828277
SP P43146
TPG DAA15913
AlphaFold P43146

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks