BMRB Entry 11134

Title:
Solution structure of the HMG box domain from human WD repeat and HMG-box DNA binding protein 1
Deposition date:
2010-03-31
Original release date:
2011-04-01
Authors:
Tomizawa, T.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.. "Solution structure of the HMG box domain from human WD repeat and HMG-box DNA binding protein 1"  .

Assembly members:

Assembly members:
HMG box domain, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050111-08

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts76
1H chemical shifts490

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HMG box domain1

Entities:

Entity 1, HMG box domain 81 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGPROLYS
2   THRGLYPHEGLNMETTRPLEUGLUGLUASN
3   ARGSERASNILELEUSERASPASNPROASP
4   PHESERASPGLUALAASPILEILELYSGLU
5   GLYMETILEARGPHEARGVALLEUSERTHR
6   GLUGLUARGLYSVALTRPALAASNLYSALA
7   LYSGLYGLUTHRALASERGLUGLYTHRGLU
8   ALALYSLYSARGLYSSERGLYPROSERSER
9   GLY

Samples:

sample_1: HMG box domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAF85694 BAG50934 BAG50944 BAG50951
EMBL CAA06932
GB AAH63041 EAW80654
REF NP_001008397 NP_009017 XP_001161792 XP_003831741 XP_004055246
SP O75717
AlphaFold O75717

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks