BMRB Entry 11178

Title:
Solution structure of the SH3 domain of human olygophrein-1 like protein (KIAA0621)
Deposition date:
2010-07-21
Original release date:
2011-07-20
Authors:
Inoue, K.; Hayashi, F.; Shirouzu, M.; Terada, T.; Kigawa, T.; Inoue, M.; Yabuki, T.; Aoki, M.; Seki, E.; Matsuda, T.; Hirota, H.; Yoshida, M.; Tanaka, A.; Osanai, T.; Matsuo, Y.; Ohara, O.; Nagase, T.; Kikuno, R.; Nakayama, M.; Yokoyama, S.
Citation:

Citation: Inoue, K.; Hayashi, F.; Shirouzu, M.; Terada, T.; Kigawa, T.; Inoue, M.; Yabuki, T.; Aoki, M.; Seki, E.; Matsuda, T.; Hirota, H.; Yoshida, M.; Tanaka, A.; Osanai, T.; Matsuo, Y.; Ohara, O.; Nagase, T.; Kikuno, R.; Nakayama, M.; Yokoyama, S.. "Solution structure of the SH3 domain of human olygophrein-1 like protein (KIAA0621)"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P021021-18

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts280
15N chemical shifts62
1H chemical shifts432

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 72 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRPROPHE
2   ARGLYSALALYSALALEUTYRALACYSLYS
3   ALAGLUHISASPSERGLULEUSERPHETHR
4   ALAGLYTHRVALPHEASPASNVALHISPRO
5   SERGLNGLUPROGLYTRPLEUGLUGLYTHR
6   LEUASNGLYLYSTHRGLYLEUILEPROGLU
7   ASNTYRVALGLUPHELEUSERGLYPROSER
8   SERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.2 mM; Tris 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA31596 BAG60864
EMBL CAA71414
GB AAD39482 AAH68555 AIC56005 EAW61876 EAW61877
REF NP_001129080 NP_001192451 NP_055886 XP_001096464 XP_001154393
SP Q9UNA1
TPG DAA27326
AlphaFold Q9UNA1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks