BMRB Entry 11210

Title:
Solution structure of the first PDZ domain of amyloid beta A4 precursor protein-binding family A, member 1
Deposition date:
2010-07-22
Original release date:
2011-07-21
Authors:
Qin, X.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first PDZ domain of amyloid beta A4 precursor protein-binding family A, member 1"  .

Assembly members:

Assembly members:
PDZ domain, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050118-02

Data sets:
Data typeCount
13C chemical shifts406
15N chemical shifts97
1H chemical shifts671

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain of amyloid beta (A4) precursor protein-binding, family A, member 1 (X11)1

Entities:

Entity 1, PDZ domain of amyloid beta (A4) precursor protein-binding, family A, member 1 (X11) 98 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPVALPHE
2   ILEGLULYSGLNLYSGLYGLUILELEUGLY
3   VALVALILEVALGLUSERGLYTRPGLYSER
4   ILELEUPROTHRVALILEILEALAASNMET
5   METHISGLYGLYPROALAGLULYSSERGLY
6   LYSLEUASNILEGLYASPGLNILEMETSER
7   ILEASNGLYTHRSERLEUVALGLYLEUPRO
8   LEUSERTHRCYSGLNSERILEILELYSGLY
9   LEULYSASNGLNSERARGVALLYSLEUASN
10   ILEVALSERGLYPROSERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.55 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio F.r - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.9296, Kobayashi N. - data analysis

CYANA v2.0.17, Guntert P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAE28008 BAE28031
GB AAC05303 AAC39766 AAI41182 AAI56048 AAI57031
REF NP_001154 NP_113967 NP_796008 XP_001138890 XP_001365486
SP B2RUJ5 O35430 Q02410
AlphaFold B2RUJ5 Q02410 O35430

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks