BMRB Entry 11214

Title:
Solution structure of the SH3 domain of Fyn-related kinase
Deposition date:
2010-07-22
Original release date:
2011-07-21
Authors:
Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain of Fyn-related kinase"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050425-22

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts79
1H chemical shifts488

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain of Fyn-related kinase1

Entities:

Entity 1, SH3 domain of Fyn-related kinase 77 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNTYRPHE
2   VALALALEUPHEASPTYRGLNALAARGTHR
3   ALAGLUASPLEUSERPHEARGALAGLYASP
4   LYSLEUGLNVALLEUASPTHRSERHISGLU
5   GLYTRPTRPLEUALAARGHISLEUGLULYS
6   LYSGLYTHRGLYLEUGLYGLNGLNLEUGLN
7   GLYTYRILEPROSERASNTYRVALALAGLU
8   ASPSERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

Delta NMR v4.3, JEOL - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • JEOL ECA 800 MHz

Related Database Links:

PDB
DBJ BAC26155 BAC35063 BAC36718 BAE38741
EMBL CAA88658
GB AAA65197 AAH07137 EDL04891 EDL04892 EDL04893
REF NP_001153016 NP_034367 XP_006512597 XP_006512598 XP_006512599
SP Q922K9
AlphaFold Q922K9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks