BMRB Entry 11221

Title:
Solution structure of the Ig-like domain (615-713) from human Obscurin-like protein 1
Deposition date:
2010-07-22
Original release date:
2011-07-21
Authors:
Qin, X.; Suetake, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Suetake, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the Ig-like domain (615-713) from human Obscurin-like protein 1"  .

Assembly members:

Assembly members:
Ig-like domain, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050725-17

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts112
1H chemical shifts721

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ig-like domain of Obscurin-like protein 11

Entities:

Entity 1, Ig-like domain of Obscurin-like protein 1 112 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRALAARG
2   LEUVALALAGLYLEUGLUASPVALGLNVAL
3   TYRASPGLYGLUASPALAVALPHESERLEU
4   ASPLEUSERTHRILEILEGLNGLYTHRTRP
5   PHELEUASNGLYGLUGLULEULYSSERASN
6   GLUPROGLUGLYGLNVALGLUPROGLYALA
7   LEUARGTYRARGILEGLUGLNLYSGLYLEU
8   GLNHISARGLEUILELEUHISALAVALLYS
9   HISGLNASPSERGLYALALEUVALGLYPHE
10   SERCYSPROGLYVALGLNASPSERALAALA
11   LEUTHRILEGLNGLUSERSERGLYPROSER
12   SERGLY

Samples:

sample_1: Ig-like domain, [U-13C; U-15N], 1.10 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9816, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks