BMRB Entry 16187

Title:
Backbone 1H Chemical Shift Assignments for FP1
Deposition date:
2009-02-26
Original release date:
2009-04-17
Authors:
Araki, Mitsugu; Tamura, Atsuo
Citation:

Citation: Araki, Mitsugu; Tamura, Atsuo. "Solubility-dependent structural formation of a 25-residue natively unfolded protein, induced by addition of a seven-residue peptide fragment"  FEBS J. 276, 2336-2347 (2009).
PubMed: 19292861

Assembly members:

Assembly members:
FP1, polymer, 32 residues, 3519.278 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FP1: YAFACPACPKRFMRSDALSK HIKTAFIVVALG

Data sets:
Data typeCount
1H chemical shifts225

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FP11

Entities:

Entity 1, FP1 32 residues - 3519.278 Da.

1   TYRALAPHEALACYSPROALACYSPROLYS
2   ARGPHEMETARGSERASPALALEUSERLYS
3   HISILELYSTHRALAPHEILEVALVALALA
4   LEUGLY

Samples:

sample_1: FP1 3 mM; acetic acid, [U-100% 2H], 25 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 3.0; pressure: 1 atm; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

DYANA v1.5, Guntert, Braun and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

PDB