BMRB Entry 16634

Title:
Backbone Dynamics and Global Effects of an Activity Enhancing Mutation in Minimized Mtu RecA Inteins
Deposition date:
2009-12-15
Original release date:
2010-06-28
Authors:
Du, Zhenming; Liu, Yangzhong; Ban, David; Lopez, Maria; Belfort, Marlene; Wang, Chunyu
Citation:

Citation: Du, Zhenming; Liu, Yangzhong; Ban, David; Lopez, Maria; Belfort, Marlene; Wang, Chunyu. "Backbone dynamics and global effects of an activating mutation in minimized Mtu RecA inteins."  J. Mol. Biol. 400, 755-767 (2010).
PubMed: 20562025

Assembly members:

Assembly members:
Mtu_RecA_intein, polymer, 139 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pXI

Data sets:
Data typeCount
15N chemical shifts132
1H chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ddIhh-V67CM1

Entities:

Entity 1, ddIhh-V67CM 139 residues - Formula weight is not available

1   CYSLEUALAGLUGLYTHRARGILEPHEASP
2   PROVALTHRGLYTHRTHRHISARGILEGLU
3   ASPVALVALGLYGLYARGLYSPROILEHIS
4   VALVALALAALAALALYSASPGLYTHRLEU
5   HISALAARGPROVALVALSERTRPPHEASP
6   GLNGLYTHRARGASPVALILEGLYLEUARG
7   ILEALAGLYGLYALAILEVALTRPALATHR
8   PROASPHISLYSVALLEUTHRGLUTYRGLY
9   TRPARGALAALAGLYGLULEUARGLYSGLY
10   ASPARGVALALAVALARGASPVALGLUTHR
11   GLYGLULEUARGTYRSERVALILEARGGLU
12   VALLEUPROTHRARGARGALAARGTHRPHE
13   GLYLEUGLUVALGLUGLULEUHISTHRLEU
14   VALALAGLUGLYVALVALVALHISASN

Samples:

sample_1: Mtu RecA intein, [U-99% 13C; U-99% 15N], 0.15 – 0.3 mM; H2O 90%; D2O 10%; NaH2PO4/Na2HPO4 50 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15560 17414
PDB
EMBL CFR77613 CMM98736
GB EPZ65824 KAM73204
REF WP_049963343

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks