BMRB Entry 16921

Title:
1H, 13C, 15N chemical shifts for the bacterial cell division regulator MinE.
Deposition date:
2010-05-11
Original release date:
2010-07-26
Authors:
Ducat, Thierry; Goto, Natalie
Citation:

Citation: Ducat, Thierry; Goto, Natalie. "1H, 13C, 15N chemical shift assignments for the Neisseria gonorrhoeae MinE regulator of cell division septum placement."  Biomol. NMR Assignments 4, 227-229 (2010).
PubMed: 20623208

Assembly members:

Assembly members:
MinE, polymer, 95 residues, 11048 Da.

Natural source:

Natural source:   Common Name: Neisseria gonorrhoeae   Taxonomy ID: 485   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria gonorrhoeae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEE46A

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts95
1H chemical shifts640

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MinE, chain 11
2MinE, chain 21

Entities:

Entity 1, MinE, chain 1 95 residues - 11048 Da.

Two non-native residues (introduced from sub-cloning) plus 6 histidine residues (for purification) included at the C-terminus of the MinE sequence from N. gonorrhoeae.

1   METSERLEUILEGLULEULEUPHEGLYARG
2   LYSGLNLYSTHRALATHRVALALAARGASP
3   ARGLEUGLNILEILEILEALAGLNGLUARG
4   ALAGLNGLUGLYGLNTHRPROASPTYRLEU
5   PROTHRLEUARGLYSALALEUMETGLUVAL
6   LEUSERLYSTYRVALASNVALSERLEUASP
7   ASNILEARGILESERGLNGLULYSGLNASP
8   GLYMETASPVALLEUGLULEUASNILETHR
9   LEUPROGLUGLNLYSLYSVALLEUGLUHIS
10   HISHISHISHISHIS

Samples:

sample_1: MinE, [U-100% 13C; U-100% 15N], 0.8 – 1.0 mM; sodium chloride 45 mM; TRIS 22.5 mM; H2O 90%; D2O 10%

sample_2: MinE, [U-100% 13C; U-100% 15N], 0.6 mM; sodium chloride 50 mM; TRIS 25 mM; D2O 100%

sample_conditions_1: ionic strength: 0.045 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.050 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_2
3D HCCH-TOCSYsample_2isotropicsample_conditions_2
3D 1H-15N TOCSY-HSQCsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_2isotropicsample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, chemical shift calculation

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
EMBL CAM07417 CEZ97582 CFA84494 CFB79676 CFC32949
GB AAK30127 AAW90432 ACF31058 AKP11296 AKP13283
REF WP_002246740 WP_003690052 WP_003749826 YP_208844
SP P58152 Q5F5V5 Q9JX18
AlphaFold P58152 Q5F5V5 Q9JX18

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks