BMRB Entry 17043

Title:
TMS2 domain of Dengue virus NS4A protein
Deposition date:
2010-07-06
Original release date:
2014-03-05
Authors:
Montserret, Roland; Bartenschlager, Ralf; Penin, Francois
Citation:

Citation: Montserret, Roland; Penin, Francois; Bartenschlager, Ralf. "TMS2 domain of Dengue virus NS4A protein"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
TMS2, polymer, 34 residues, 3661.534 Da.

Natural source:

Natural source:   Common Name: Dengue virus   Taxonomy ID: 12637   Superkingdom: not available   Kingdom: not available   Genus/species: Flavivirus not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TMS2: KKSGRGIGKMTLGMCCIITA SILLWYAQIQGGKK

Data sets:
Data typeCount
13C chemical shifts96
1H chemical shifts221

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TMS2 domain1

Entities:

Entity 1, TMS2 domain 34 residues - 3661.534 Da.

two solubilisation tag added to the sequence ; KK in N terminus and GGKK in C terminus

1   LYSLYSSERGLYARGGLYILEGLYLYSMET
2   THRLEUGLYMETCYSCYSILEILETHRALA
3   SERILELEULEUTRPTYRALAGLNILEGLN
4   GLYGLYLYSLYS

Samples:

sample_1: entity 1 ± 0.1 mM; trifluoroethanol, [U-99% 2H], 50 ± 1 % v/v; H2O 50 ± 1 % v/v; DTT, [U-99% 2H], 20 ± 1 mM; DSS 0.2 ± 0.05 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

X-PLOR NIH v2.24, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

DBJ BAJ05336 BAJ05337 BAJ05338
EMBL CAD31751 CAI92123 CAR65136 CAR65137 CAR65138
GB AAA42941 AAA42962 AAA73185 AAB58782 AAC40832
PRF 1301272B
REF NP_056776 NP_739588
SP P12823 P14340 P29990 Q9WDA6
AlphaFold P12823 P14340 P29990 Q9WDA6